Spermidine labels proteins during sea urchin embryogenesis

Z. N. Canellakis; L. L. Marsh; Y. C. Manabe; A. A. Infante; P. K. Bondy; F. W. Scalise

Spermidine labels proteins during sea urchin embryogenesis

Z. N. Canellakis, L. L. Marsh, Y. C. Manabe, A. A. Infante, P. K. Bondy, F. W. Scalise

Research output: Contribution to journal › Article › peer-review

Abstract

We have previously described the presence of a protein containing intact, covalently bound spermidine during very early embryogenesis of the sea urchin (Strongylocentrotus purpuratus). Proteins containing other polyamine metabolites also appear as embryogenesis proceeds. These proteins which contain label derived from exogenous radioactive spermidine show a characteristic pattern which changes during the course of embryonic development. We document for the first time that hypusine, the polyamine metabolite which is a characteristic component of the eukaryotic protein translation initiation factor eIF-4D, is present in more than one species of macromolecule. In addition, N¹-acetylspermidine has also been identified as a significant intracellular metabolite of spermidine during embryogenesis.

Original language	English (US)
Pages (from-to)	969-976
Number of pages	8
Journal	Biochemistry International
Volume	19
Issue number	5
State	Published - 1989
Externally published	Yes

ASJC Scopus subject areas

Biochemistry

Cite this

@article{c701ab1a04bb494e9fc0c7f42c559abd,

title = "Spermidine labels proteins during sea urchin embryogenesis",

abstract = "We have previously described the presence of a protein containing intact, covalently bound spermidine during very early embryogenesis of the sea urchin (Strongylocentrotus purpuratus). Proteins containing other polyamine metabolites also appear as embryogenesis proceeds. These proteins which contain label derived from exogenous radioactive spermidine show a characteristic pattern which changes during the course of embryonic development. We document for the first time that hypusine, the polyamine metabolite which is a characteristic component of the eukaryotic protein translation initiation factor eIF-4D, is present in more than one species of macromolecule. In addition, N1-acetylspermidine has also been identified as a significant intracellular metabolite of spermidine during embryogenesis.",

author = "Canellakis, {Z. N.} and Marsh, {L. L.} and Manabe, {Y. C.} and Infante, {A. A.} and Bondy, {P. K.} and Scalise, {F. W.}",

year = "1989",

language = "English (US)",

volume = "19",

pages = "969--976",

journal = "Biochemistry International",

issn = "0158-5231",

publisher = "John Wiley and Sons Inc.",

number = "5",

}

TY - JOUR

T1 - Spermidine labels proteins during sea urchin embryogenesis

AU - Canellakis, Z. N.

AU - Marsh, L. L.

AU - Manabe, Y. C.

AU - Infante, A. A.

AU - Bondy, P. K.

AU - Scalise, F. W.

PY - 1989

Y1 - 1989

N2 - We have previously described the presence of a protein containing intact, covalently bound spermidine during very early embryogenesis of the sea urchin (Strongylocentrotus purpuratus). Proteins containing other polyamine metabolites also appear as embryogenesis proceeds. These proteins which contain label derived from exogenous radioactive spermidine show a characteristic pattern which changes during the course of embryonic development. We document for the first time that hypusine, the polyamine metabolite which is a characteristic component of the eukaryotic protein translation initiation factor eIF-4D, is present in more than one species of macromolecule. In addition, N1-acetylspermidine has also been identified as a significant intracellular metabolite of spermidine during embryogenesis.

AB - We have previously described the presence of a protein containing intact, covalently bound spermidine during very early embryogenesis of the sea urchin (Strongylocentrotus purpuratus). Proteins containing other polyamine metabolites also appear as embryogenesis proceeds. These proteins which contain label derived from exogenous radioactive spermidine show a characteristic pattern which changes during the course of embryonic development. We document for the first time that hypusine, the polyamine metabolite which is a characteristic component of the eukaryotic protein translation initiation factor eIF-4D, is present in more than one species of macromolecule. In addition, N1-acetylspermidine has also been identified as a significant intracellular metabolite of spermidine during embryogenesis.

UR - http://www.scopus.com/inward/record.url?scp=0024451242&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024451242&partnerID=8YFLogxK

M3 - Article

C2 - 2635856

AN - SCOPUS:0024451242

SN - 0158-5231

VL - 19

SP - 969

EP - 976

JO - Biochemistry International

JF - Biochemistry International

IS - 5

ER -

Spermidine labels proteins during sea urchin embryogenesis

Abstract

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this