Spermatogonial Stem Cell Numbers Are Reduced by Transient Inhibition of GDNF Signaling but Restored by Self-Renewing Replication when Signaling Resumes

Nicole Parker; Andrew Laychur; Meena Sukwani; Kyle E. Orwig; Jon M. Oatley; Chao Zhang; Florentine U. Rutaganira; Kevan Shokat; William W. Wright

doi:10.1016/j.stemcr.2021.01.015

Spermatogonial Stem Cell Numbers Are Reduced by Transient Inhibition of GDNF Signaling but Restored by Self-Renewing Replication when Signaling Resumes

Nicole Parker, Andrew Laychur, Meena Sukwani, Kyle E. Orwig, Jon M. Oatley, Chao Zhang, Florentine U. Rutaganira, Kevan Shokat, William W. Wright

Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

Abstract

Wright and colleagues investigate the restoration of adult spermatogonial stem cells (SSCs) following 9 days of inhibited GDNF signaling. Immediately after signaling resumes, SSC replication generates progenitors. Then, self-renewing replication restores SSC numbers. Testicular GDNF levels are not increased during restoration. However, the fraction of replicating SSCs decreases as their numbers increase, suggesting that interactions among them regulate restoration.

Original language	English (US)
Pages (from-to)	597-609
Number of pages	13
Journal	Stem Cell Reports
Volume	16
Issue number	3
DOIs	https://doi.org/10.1016/j.stemcr.2021.01.015
State	Published - Mar 9 2021

Keywords

GDNF
GFRα1
Ret
Sertoli cell
blood-testis barrier
infertility
progenitor spermatogonia
spermatogonial stem cell

ASJC Scopus subject areas

Biochemistry
Genetics
Developmental Biology
Cell Biology

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10.1016/j.stemcr.2021.01.015

Cite this

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title = "Spermatogonial Stem Cell Numbers Are Reduced by Transient Inhibition of GDNF Signaling but Restored by Self-Renewing Replication when Signaling Resumes",

abstract = "Wright and colleagues investigate the restoration of adult spermatogonial stem cells (SSCs) following 9 days of inhibited GDNF signaling. Immediately after signaling resumes, SSC replication generates progenitors. Then, self-renewing replication restores SSC numbers. Testicular GDNF levels are not increased during restoration. However, the fraction of replicating SSCs decreases as their numbers increase, suggesting that interactions among them regulate restoration.",

keywords = "GDNF, GFRα1, Ret, Sertoli cell, blood-testis barrier, infertility, progenitor spermatogonia, spermatogonial stem cell",

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doi = "10.1016/j.stemcr.2021.01.015",

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AU - Parker, Nicole

AU - Laychur, Andrew

AU - Sukwani, Meena

AU - Orwig, Kyle E.

AU - Oatley, Jon M.

AU - Zhang, Chao

AU - Rutaganira, Florentine U.

AU - Shokat, Kevan

AU - Wright, William W.

PY - 2021/3/9

Y1 - 2021/3/9

N2 - Wright and colleagues investigate the restoration of adult spermatogonial stem cells (SSCs) following 9 days of inhibited GDNF signaling. Immediately after signaling resumes, SSC replication generates progenitors. Then, self-renewing replication restores SSC numbers. Testicular GDNF levels are not increased during restoration. However, the fraction of replicating SSCs decreases as their numbers increase, suggesting that interactions among them regulate restoration.

AB - Wright and colleagues investigate the restoration of adult spermatogonial stem cells (SSCs) following 9 days of inhibited GDNF signaling. Immediately after signaling resumes, SSC replication generates progenitors. Then, self-renewing replication restores SSC numbers. Testicular GDNF levels are not increased during restoration. However, the fraction of replicating SSCs decreases as their numbers increase, suggesting that interactions among them regulate restoration.

KW - GDNF

KW - GFRα1

KW - Ret

KW - Sertoli cell

KW - blood-testis barrier

KW - infertility

KW - progenitor spermatogonia

KW - spermatogonial stem cell

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Spermatogonial Stem Cell Numbers Are Reduced by Transient Inhibition of GDNF Signaling but Restored by Self-Renewing Replication when Signaling Resumes

Abstract

Keywords

ASJC Scopus subject areas

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