Spectroscopic imaging using acousto-optic tuneable filters

Mounir Bouhifd, Maurice Whelan

Research output: Chapter in Book/Report/Conference proceedingConference contribution


We report on novel hyper-spectral imaging filter-modules based on acousto-optic tuneable filters (AOTF). The AOTF functions as a full-field tuneable bandpass filter which offers fast continuous or random access tuning with high filtering efficiency. Due to the diffractive nature of the device, the unfiltered zero-order and the filtered first-order images are geometrically separated. The modules developed exploit this feature to simultaneously route both the transmitted whitelight image and the filtered fluorescence image to two separate cameras. Incorporation of prisms in the optical paths and careful design of the relay optics in the filter module have overcome a number of aberrations inherent to imaging through AOTFs, leading to excellent spatial resolution. A number of practical uses of this technique, both for in vivo auto-fluorescence endoscopy and in vitro fluorescence microscopy were demonstrated. We describe the operational principle and design of recently improved prototype instruments for fluorescence-based diagnostics and demonstrate their performance by presenting challenging hyper-spectral fluorescence imaging applications.

Original languageEnglish (US)
Title of host publicationEuropean Conference on Biomedical Optics, ECBO 2007
PublisherOptical Society of America
ISBN (Print)9780819467713
StatePublished - Jan 1 2007
EventEuropean Conference on Biomedical Optics, ECBO 2007 - Munich, Germany
Duration: Jun 17 2007Jun 17 2007

Publication series

NameOptics InfoBase Conference Papers
ISSN (Electronic)2162-2701


OtherEuropean Conference on Biomedical Optics, ECBO 2007


  • AOTF
  • Autofluorescence
  • Endoscopy
  • Microscopy
  • Spectroscopy

ASJC Scopus subject areas

  • Instrumentation
  • Atomic and Molecular Physics, and Optics


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