Specific MR imaging of human lymphocytes by monoclonal antibody‐guided dextran‐magnetite particles

Jeff W.M. Bulte; Yke Hoekstra; Richard L. Kamman; Richard L. Magin; Andrew G. Webb; Richard W. Briggs; K. Gwan Go; Caesar E. Hulstaert; Stefan Miltenyi; T. Hauw The; Lou De Leij

doi:10.1002/mrm.1910250115

Specific MR imaging of human lymphocytes by monoclonal antibody‐guided dextran‐magnetite particles

Jeff W.M. Bulte, Yke Hoekstra, Richard L. Kamman, Richard L. Magin, Andrew G. Webb, Richard W. Briggs, K. Gwan Go, Caesar E. Hulstaert, Stefan Miltenyi, T. Hauw The, Lou De Leij

Research output: Contribution to journal › Article › peer-review

114 Scopus citations

Abstract

Human lymphocytes were labeled with biotinylated anti‐lymphocyte‐directed monoclonal antibodies, to which streptavidin and subsequently biotinylated dextran‐magnetite particles were coupled. This labeling resulted in a strong and selective negative contrast enhancement of lymphocyte suspensions at 2.0 T, caused predominantly by the specific increase of R₂ with a small but significant specific increase of R₂. The R₁ was found to decrease with increasing field strength. The immunolabeling procedure described here may be used for the selective signal depletion of target cells in MR imaging.

Original language	English (US)
Pages (from-to)	148-157
Number of pages	10
Journal	Magnetic resonance in medicine
Volume	25
Issue number	1
DOIs	https://doi.org/10.1002/mrm.1910250115
State	Published - May 1992
Externally published	Yes

ASJC Scopus subject areas

Radiology Nuclear Medicine and imaging

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abstract = "Human lymphocytes were labeled with biotinylated anti‐lymphocyte‐directed monoclonal antibodies, to which streptavidin and subsequently biotinylated dextran‐magnetite particles were coupled. This labeling resulted in a strong and selective negative contrast enhancement of lymphocyte suspensions at 2.0 T, caused predominantly by the specific increase of R2 with a small but significant specific increase of R2. The R1 was found to decrease with increasing field strength. The immunolabeling procedure described here may be used for the selective signal depletion of target cells in MR imaging.",

author = "Bulte, {Jeff W.M.} and Yke Hoekstra and Kamman, {Richard L.} and Magin, {Richard L.} and Webb, {Andrew G.} and Briggs, {Richard W.} and {Gwan Go}, K. and Hulstaert, {Caesar E.} and Stefan Miltenyi and {Hauw The}, T. and {De Leij}, Lou",

year = "1992",

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doi = "10.1002/mrm.1910250115",

language = "English (US)",

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T1 - Specific MR imaging of human lymphocytes by monoclonal antibody‐guided dextran‐magnetite particles

AU - Bulte, Jeff W.M.

AU - Hoekstra, Yke

AU - Kamman, Richard L.

AU - Magin, Richard L.

AU - Webb, Andrew G.

AU - Briggs, Richard W.

AU - Gwan Go, K.

AU - Hulstaert, Caesar E.

AU - Miltenyi, Stefan

AU - Hauw The, T.

AU - De Leij, Lou

PY - 1992/5

Y1 - 1992/5

N2 - Human lymphocytes were labeled with biotinylated anti‐lymphocyte‐directed monoclonal antibodies, to which streptavidin and subsequently biotinylated dextran‐magnetite particles were coupled. This labeling resulted in a strong and selective negative contrast enhancement of lymphocyte suspensions at 2.0 T, caused predominantly by the specific increase of R2 with a small but significant specific increase of R2. The R1 was found to decrease with increasing field strength. The immunolabeling procedure described here may be used for the selective signal depletion of target cells in MR imaging.

AB - Human lymphocytes were labeled with biotinylated anti‐lymphocyte‐directed monoclonal antibodies, to which streptavidin and subsequently biotinylated dextran‐magnetite particles were coupled. This labeling resulted in a strong and selective negative contrast enhancement of lymphocyte suspensions at 2.0 T, caused predominantly by the specific increase of R2 with a small but significant specific increase of R2. The R1 was found to decrease with increasing field strength. The immunolabeling procedure described here may be used for the selective signal depletion of target cells in MR imaging.

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Specific MR imaging of human lymphocytes by monoclonal antibody‐guided dextran‐magnetite particles

Abstract

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