Abstract
The ability to create and maintain neuron and glial cell co-cultures is important for neuronal regeneration as well as for fundamental studies on neuron and glial cell interactions. We demonstrate here a method for spatially controlling the arrangement of neurons and glial cells. Line patterns of cell resistant, poly(oligoethyleneglycol methacrylate-co-methacrylic acid), was microcontact printed on various substrates to spatially control the attachment of neurons. Neuron-like cells, PC12 and SH-SY5Y cells, were confined within the unprinted line patterns and extended neurites along the line patterns. Subsequent attachment of glial cells was accomplished by converting the originally cell-resistant line patterns of poly(oligoethyleneglycol methacrylate-co-methacrylic acid) to cell adhesive by electrostatic adsorption of cationic poly-lysine, chitosan, or poly-(ethyleneimine). This method for creating patterned co-cultures of neuron and glial cells provides a useful tool for investigating neuron-glial cell interactions and has potential applications in the repair or regeneration of nervous systems.
Original language | English (US) |
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Pages (from-to) | 976-984 |
Number of pages | 9 |
Journal | Journal of Biomedical Materials Research - Part A |
Volume | 75 |
Issue number | 4 |
DOIs | |
State | Published - Dec 15 2005 |
Keywords
- Chitosan
- Co-culture
- Glial cell
- Neuron
- Poly(OEGMA-co-MA)
- Poly-L-lysine
ASJC Scopus subject areas
- Ceramics and Composites
- Biomaterials
- Biomedical Engineering
- Metals and Alloys