Spatially controlled co-culture of neurons and glial cells

In Hong Yang, Carlos C. Co, Chia Chi Ho

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


The ability to create and maintain neuron and glial cell co-cultures is important for neuronal regeneration as well as for fundamental studies on neuron and glial cell interactions. We demonstrate here a method for spatially controlling the arrangement of neurons and glial cells. Line patterns of cell resistant, poly(oligoethyleneglycol methacrylate-co-methacrylic acid), was microcontact printed on various substrates to spatially control the attachment of neurons. Neuron-like cells, PC12 and SH-SY5Y cells, were confined within the unprinted line patterns and extended neurites along the line patterns. Subsequent attachment of glial cells was accomplished by converting the originally cell-resistant line patterns of poly(oligoethyleneglycol methacrylate-co-methacrylic acid) to cell adhesive by electrostatic adsorption of cationic poly-lysine, chitosan, or poly-(ethyleneimine). This method for creating patterned co-cultures of neuron and glial cells provides a useful tool for investigating neuron-glial cell interactions and has potential applications in the repair or regeneration of nervous systems.

Original languageEnglish (US)
Pages (from-to)976-984
Number of pages9
JournalJournal of Biomedical Materials Research - Part A
Issue number4
StatePublished - Dec 15 2005


  • Chitosan
  • Co-culture
  • Glial cell
  • Neuron
  • Poly(OEGMA-co-MA)
  • Poly-L-lysine

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys


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