Spatially compartmentalized phase regulation of a ca2+-camp-pka oscillatory circuit

Brian Tenner, Michael Getz, Brian Ross, Donya Ohadi, Christopher H. Bohrer, Eric Greenwald, Sohum Mehta, Jie Xiao, Padmini Rangamani, Jin Zhang

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Signaling networks are spatiotemporally organized to sense diverse inputs, process information, and carry out specific cellular tasks. In b cells, Ca2+, cyclic adenosine monophosphate (cAMP), and Protein Kinase A (PKA) exist in an oscillatory circuit characterized by a high degree of feedback. Here, we describe a mode of regulation within this circuit involving a spatial dependence of the relative phase between cAMP, PKA, and Ca2+. We show that in mouse MIN6 b cells, nanodomain clustering of Ca2+-sensitive adenylyl cyclases (ACs) drives oscillations of local cAMP levels to be precisely in-phase with Ca2+ oscillations, whereas Ca2+-sensitive phosphodiesterases maintain out-of-phase oscillations outside of the nanodomain. Disruption of this precise phase relationship perturbs Ca2+ oscillations, suggesting the relative phase within an oscillatory circuit can encode specific functional information. This work unveils a novel mechanism of cAMP compartmentation utilized for localized tuning of an oscillatory circuit and has broad implications for the spatiotemporal regulation of signaling networks.

Original languageEnglish (US)
Article numbere55013
Pages (from-to)1-34
Number of pages34
StatePublished - Oct 2020

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)


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