Spatial localization of the Ebola virus glycoprotein mucin-like domain determined by cryo-electron tomography

Erin E H Tran; James A. Simmons; Alberto Bartesaghi; Charles J. Shoemaker; Elizabeth Nelson; Judith M. White; Sriram Subramaniam

doi:10.1128/JVI.00870-14

Spatial localization of the Ebola virus glycoprotein mucin-like domain determined by cryo-electron tomography

Erin E H Tran, James A. Simmons, Alberto Bartesaghi, Charles J. Shoemaker, Elizabeth Nelson, Judith M. White, Sriram Subramaniam

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

The Ebola virus glycoprotein mucin-like domain (MLD) is implicated in Ebola virus cell entry and immune evasion. Using cryo-electron tomography of Ebola virus-like particles, we determined a three-dimensional structure for the full-length glycoprotein in a near-native state and compared it to that of a glycoprotein lacking the MLD. Our results, which show that the MLD is located at the apex and the sides of each glycoprotein monomer, provide a structural template for analysis of MLD function.

Original language	English (US)
Pages (from-to)	10958-10962
Number of pages	5
Journal	Journal of Virology
Volume	88
Issue number	18
DOIs	https://doi.org/10.1128/JVI.00870-14
State	Published - 2014
Externally published	Yes

ASJC Scopus subject areas

Immunology
Virology
Medicine(all)

Access to Document

10.1128/JVI.00870-14

Cite this

@article{12a7954847fd4f45a7d7034b64cf9336,

title = "Spatial localization of the Ebola virus glycoprotein mucin-like domain determined by cryo-electron tomography",

abstract = "The Ebola virus glycoprotein mucin-like domain (MLD) is implicated in Ebola virus cell entry and immune evasion. Using cryo-electron tomography of Ebola virus-like particles, we determined a three-dimensional structure for the full-length glycoprotein in a near-native state and compared it to that of a glycoprotein lacking the MLD. Our results, which show that the MLD is located at the apex and the sides of each glycoprotein monomer, provide a structural template for analysis of MLD function.",

author = "Tran, {Erin E H} and Simmons, {James A.} and Alberto Bartesaghi and Shoemaker, {Charles J.} and Elizabeth Nelson and White, {Judith M.} and Sriram Subramaniam",

year = "2014",

doi = "10.1128/JVI.00870-14",

language = "English (US)",

volume = "88",

pages = "10958--10962",

journal = "Journal of Virology",

issn = "0022-538X",

publisher = "American Society for Microbiology",

number = "18",

}

TY - JOUR

T1 - Spatial localization of the Ebola virus glycoprotein mucin-like domain determined by cryo-electron tomography

AU - Tran, Erin E H

AU - Simmons, James A.

AU - Bartesaghi, Alberto

AU - Shoemaker, Charles J.

AU - Nelson, Elizabeth

AU - White, Judith M.

AU - Subramaniam, Sriram

PY - 2014

Y1 - 2014

N2 - The Ebola virus glycoprotein mucin-like domain (MLD) is implicated in Ebola virus cell entry and immune evasion. Using cryo-electron tomography of Ebola virus-like particles, we determined a three-dimensional structure for the full-length glycoprotein in a near-native state and compared it to that of a glycoprotein lacking the MLD. Our results, which show that the MLD is located at the apex and the sides of each glycoprotein monomer, provide a structural template for analysis of MLD function.

AB - The Ebola virus glycoprotein mucin-like domain (MLD) is implicated in Ebola virus cell entry and immune evasion. Using cryo-electron tomography of Ebola virus-like particles, we determined a three-dimensional structure for the full-length glycoprotein in a near-native state and compared it to that of a glycoprotein lacking the MLD. Our results, which show that the MLD is located at the apex and the sides of each glycoprotein monomer, provide a structural template for analysis of MLD function.

UR - http://www.scopus.com/inward/record.url?scp=84906346444&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84906346444&partnerID=8YFLogxK

U2 - 10.1128/JVI.00870-14

DO - 10.1128/JVI.00870-14

M3 - Article

C2 - 25008940

AN - SCOPUS:84906346444

SN - 0022-538X

VL - 88

SP - 10958

EP - 10962

JO - Journal of Virology

JF - Journal of Virology

IS - 18

ER -

Spatial localization of the Ebola virus glycoprotein mucin-like domain determined by cryo-electron tomography

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this