SOX17 methylation inhibits its antagonism of Wnt signaling pathway in lung cancer.

Dongtao Yin; Yan Jia; Yuanzi Yu; Malcolm V. Brock; James G. Herman; Chao Han; Xiaomo Su; Yang Liu; Mingzhou Guo

SOX17 methylation inhibits its antagonism of Wnt signaling pathway in lung cancer.

Dongtao Yin, Yan Jia, Yuanzi Yu, Malcolm V. Brock, James G. Herman, Chao Han, Xiaomo Su, Yang Liu, Mingzhou Guo

School of Medicine

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

The purpose of this study was to explore epigenetic changes and functions of SOX17 in human lung cancer. Five lung cancer cell lines and 88 primary lung cancer samples were examined in this study. Methylation-specific polymerase chain reaction (MSP), semi-quantitative reverse-transcription PCR, immunohistochemistry, luciferase reporter assays, colony-formation assays, and western blotting were used to analyze methylation changes and functions of SOX17 in lung cancer. SOX17 methylation was found in 60.2% of primary human lung cancer samples, and promoter region methylation of SOX17 silenced its expression. SOX17 methylation was associated with female patients and lung cancer differentiation. Colony-formation assays revealed that SOX17 suppressed lung cancer cell proliferation. Re-expression of SOX17 inhibited Wnt signaling in H23 lung cancer cell line. SOX17 acts as a Wnt signaling inhibitor.

Original language	English (US)
Pages (from-to)	33-40
Number of pages	8
Journal	Discovery Medicine
Volume	14
Issue number	74
State	Published - Jul 2012

ASJC Scopus subject areas

General Medicine

Cite this

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title = "SOX17 methylation inhibits its antagonism of Wnt signaling pathway in lung cancer.",

abstract = "The purpose of this study was to explore epigenetic changes and functions of SOX17 in human lung cancer. Five lung cancer cell lines and 88 primary lung cancer samples were examined in this study. Methylation-specific polymerase chain reaction (MSP), semi-quantitative reverse-transcription PCR, immunohistochemistry, luciferase reporter assays, colony-formation assays, and western blotting were used to analyze methylation changes and functions of SOX17 in lung cancer. SOX17 methylation was found in 60.2% of primary human lung cancer samples, and promoter region methylation of SOX17 silenced its expression. SOX17 methylation was associated with female patients and lung cancer differentiation. Colony-formation assays revealed that SOX17 suppressed lung cancer cell proliferation. Re-expression of SOX17 inhibited Wnt signaling in H23 lung cancer cell line. SOX17 acts as a Wnt signaling inhibitor.",

author = "Dongtao Yin and Yan Jia and Yuanzi Yu and Brock, {Malcolm V.} and Herman, {James G.} and Chao Han and Xiaomo Su and Yang Liu and Mingzhou Guo",

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T1 - SOX17 methylation inhibits its antagonism of Wnt signaling pathway in lung cancer.

AU - Yin, Dongtao

AU - Jia, Yan

AU - Yu, Yuanzi

AU - Brock, Malcolm V.

AU - Herman, James G.

AU - Han, Chao

AU - Su, Xiaomo

AU - Liu, Yang

AU - Guo, Mingzhou

PY - 2012/7

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AB - The purpose of this study was to explore epigenetic changes and functions of SOX17 in human lung cancer. Five lung cancer cell lines and 88 primary lung cancer samples were examined in this study. Methylation-specific polymerase chain reaction (MSP), semi-quantitative reverse-transcription PCR, immunohistochemistry, luciferase reporter assays, colony-formation assays, and western blotting were used to analyze methylation changes and functions of SOX17 in lung cancer. SOX17 methylation was found in 60.2% of primary human lung cancer samples, and promoter region methylation of SOX17 silenced its expression. SOX17 methylation was associated with female patients and lung cancer differentiation. Colony-formation assays revealed that SOX17 suppressed lung cancer cell proliferation. Re-expression of SOX17 inhibited Wnt signaling in H23 lung cancer cell line. SOX17 acts as a Wnt signaling inhibitor.

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SOX17 methylation inhibits its antagonism of Wnt signaling pathway in lung cancer.

Abstract

ASJC Scopus subject areas

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