Sources of human schwann cells and the influence of donor age

Philip J. Boyer; Gerald F. Tuite; Robert C. Dauser; Karin M. Muraszko; Gihan I. Tennekoon; J. Lynn Rutkowski

doi:10.1006/exnr.1994.1184

Sources of human schwann cells and the influence of donor age

Philip J. Boyer, Gerald F. Tuite, Robert C. Dauser, Karin M. Muraszko, Gihan I. Tennekoon, J. Lynn Rutkowski

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

We evaluated several tissues as possible sources for culturing human Schwann cells. The average cell yield (total cell number/mg of nerve fascicle) obtained from adult autopsy cases and transplant organ donors was similar (2 × 10⁴ and 2.9 × 10⁴, respectively), but significantly higher yields were obtained from dorsal roots of pediatric patients undergoing selective dorsal rhizotomy (6.1 × 10⁴). Fresh tissue was not essential since cells isolated from 0 to 20 h postmortem were equally viable. However, we found evidence that donor age affects the intrinsic growth rate of Schwann cells and perineurial fibroblasts in culture.

Original language	English (US)
Pages (from-to)	53-55
Number of pages	3
Journal	Experimental Neurology
Volume	130
Issue number	1
DOIs	https://doi.org/10.1006/exnr.1994.1184
State	Published - Nov 1994
Externally published	Yes

ASJC Scopus subject areas

Neurology
Developmental Neuroscience

Access to Document

10.1006/exnr.1994.1184

Cite this

@article{8626f3d974fe48b68fbd1074ae1d82b6,

title = "Sources of human schwann cells and the influence of donor age",

abstract = "We evaluated several tissues as possible sources for culturing human Schwann cells. The average cell yield (total cell number/mg of nerve fascicle) obtained from adult autopsy cases and transplant organ donors was similar (2 × 104 and 2.9 × 104, respectively), but significantly higher yields were obtained from dorsal roots of pediatric patients undergoing selective dorsal rhizotomy (6.1 × 104). Fresh tissue was not essential since cells isolated from 0 to 20 h postmortem were equally viable. However, we found evidence that donor age affects the intrinsic growth rate of Schwann cells and perineurial fibroblasts in culture.",

author = "Boyer, {Philip J.} and Tuite, {Gerald F.} and Dauser, {Robert C.} and Muraszko, {Karin M.} and Tennekoon, {Gihan I.} and Rutkowski, {J. Lynn}",

year = "1994",

month = nov,

doi = "10.1006/exnr.1994.1184",

language = "English (US)",

volume = "130",

pages = "53--55",

journal = "Experimental Neurology",

issn = "0014-4886",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Sources of human schwann cells and the influence of donor age

AU - Boyer, Philip J.

AU - Tuite, Gerald F.

AU - Dauser, Robert C.

AU - Muraszko, Karin M.

AU - Tennekoon, Gihan I.

AU - Rutkowski, J. Lynn

PY - 1994/11

Y1 - 1994/11

N2 - We evaluated several tissues as possible sources for culturing human Schwann cells. The average cell yield (total cell number/mg of nerve fascicle) obtained from adult autopsy cases and transplant organ donors was similar (2 × 104 and 2.9 × 104, respectively), but significantly higher yields were obtained from dorsal roots of pediatric patients undergoing selective dorsal rhizotomy (6.1 × 104). Fresh tissue was not essential since cells isolated from 0 to 20 h postmortem were equally viable. However, we found evidence that donor age affects the intrinsic growth rate of Schwann cells and perineurial fibroblasts in culture.

AB - We evaluated several tissues as possible sources for culturing human Schwann cells. The average cell yield (total cell number/mg of nerve fascicle) obtained from adult autopsy cases and transplant organ donors was similar (2 × 104 and 2.9 × 104, respectively), but significantly higher yields were obtained from dorsal roots of pediatric patients undergoing selective dorsal rhizotomy (6.1 × 104). Fresh tissue was not essential since cells isolated from 0 to 20 h postmortem were equally viable. However, we found evidence that donor age affects the intrinsic growth rate of Schwann cells and perineurial fibroblasts in culture.

UR - http://www.scopus.com/inward/record.url?scp=0028558498&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028558498&partnerID=8YFLogxK

U2 - 10.1006/exnr.1994.1184

DO - 10.1006/exnr.1994.1184

M3 - Article

C2 - 7821396

AN - SCOPUS:0028558498

SN - 0014-4886

VL - 130

SP - 53

EP - 55

JO - Experimental Neurology

JF - Experimental Neurology

IS - 1

ER -

Sources of human schwann cells and the influence of donor age

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this