Sorting nexin 27 regulates basal and stimulated brush border trafficking of NHE3

Varsha Singh, Jianbo Yang, Boyoung Cha, Tiane E. Chen, Rafiquel Sarker, Jianyi Yin, Leela Rani Avula, Ming Tse, Mark Donowitz

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


Sorting nexin 27 (SNX27) contains a PDZ domain that is phylogenetically related to the PDZ domains of the NHERF proteins. Studies on nonepithelial cells have shown that this protein is located in endosomes, where it regulates trafficking of cargo proteins in a PDZ domain-dependent manner. However, the role of SNX27 in trafficking of cargo proteins in epithelial cells has not been adequately explored. Here we show that SNX27 directly interacts with NHE3 (C-terminus) primarily through the SNX27 PDZ domain. A combination of knockdown and reconstitution experiments with wild type and a PDZ domain mutant (GYGF → GAGA) of SNX27 demonstrate that the PDZ domain of SNX27 is required to maintain basal NHE3 activity and surface expression of NHE3 in polarized epithelial cells. Biotinylation-based recycling and degradation studies in intestinal epithelial cells show that SNX27 is required for the exocytosis (not endocytosis) of NHE3 from early endosome to plasma membrane. SNX27 is also required to regulate the retention of NHE3 on the plasma membrane. The findings of the present study extend our understanding of PDZ-mediated recycling of cargo proteins from endosome to plasma membrane in epithelial cells.

Original languageEnglish (US)
Pages (from-to)2030-2043
Number of pages14
JournalMolecular biology of the cell
Issue number11
StatePublished - Jun 1 2015

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology


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