Perturbation of sarcoplasmic reticulum ATPase with the nonionic detergent C12 E8 is modulated by the amount of free Ca2+ present in the solvent prior to the addition of detergent. CD measurements show that the enzyme exists in solutionin two different conformations that react differently with the detergent. They probably represent the free enzyme, and its complex with Ca2+ On this assumption, titrations with increasing amounts of Ca2+ produced data superimposable on curves obtained measuring Ca2+ bound to sarcoplasmic reticulum vesicles.
- Sarcoplasmic reticulum: A TPase
- Solvent perturbation
ASJC Scopus subject areas
- Organic Chemistry