Solid-phase inclusion as a mechanism for regulating unfolded proteins in the mitochondrial matrix

Linhao Ruan, Joshua T. McNamara, Xi Zhang, Alexander Chih Chieh Chang, Jin Zhu, Yi Dong, Gordon Sun, Amy Peterson, Chan Hyun Na, Rong Li

Research output: Contribution to journalArticlepeer-review


Proteostasis declines with age, characterized by the accumulation of unfolded or damaged proteins. Recent studies suggest that proteins constituting pathological inclusions in neurodegenerative diseases also enter and accumulate in mitochondria. How unfolded proteins are managed within mitochondria remains unclear. Here, we found that excessive unfolded proteins in the mitochondrial matrix of yeast cells are consolidated into solid-phase inclusions, which we term deposits of unfolded mitochondrial proteins (DUMP). Formation of DUMP occurs in mitochondria near endoplasmic reticulum–mitochondria contact sites and is regulated by mitochondrial proteins controlling the production of cytidine 5′-diphosphate–diacylglycerol. DUMP formation is age dependent but accelerated by exogenous unfolded proteins. Many enzymes of the tricarboxylic acid cycle were enriched in DUMP. During yeast cell division, DUMP formation is necessary for asymmetric inheritance of damaged mitochondrial proteins between mother and daughter cells. We provide evidence that DUMP-like structures may be induced by excessive unfolded proteins in human cells.

Original languageEnglish (US)
Article numbereabc7288
JournalScience Advances
Issue number32
StatePublished - Aug 2020

ASJC Scopus subject areas

  • General


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