TY - JOUR
T1 - Solation of elastic tissue microfibrils derived from cultured cells of calf ligamentum nuchae
AU - Lamberg, Stanford I.
AU - Poppke, Donald C.
AU - Williams, Barbara R.
PY - 1980
Y1 - 1980
N2 - Cells cultured from calf ligamentum nuchae produce extracellular microfibrils identical to those of intact elastic tissue as determined by ultrastructural appearance, degradative enzyme susceptibilities and amino acid composition. A method to isolate large amounts of the microfibrillar component using fluorescein mercuric acetate is described. The cultures do not appear to synthesize the amorphous component, elastin, in that radioactive desmosine and isodesmo-sine were not detected after incubating cultures with labeled lysine nor was elastin seen ultrastructurally.
AB - Cells cultured from calf ligamentum nuchae produce extracellular microfibrils identical to those of intact elastic tissue as determined by ultrastructural appearance, degradative enzyme susceptibilities and amino acid composition. A method to isolate large amounts of the microfibrillar component using fluorescein mercuric acetate is described. The cultures do not appear to synthesize the amorphous component, elastin, in that radioactive desmosine and isodesmo-sine were not detected after incubating cultures with labeled lysine nor was elastin seen ultrastructurally.
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U2 - 10.3109/03008208009152115
DO - 10.3109/03008208009152115
M3 - Article
C2 - 6450659
AN - SCOPUS:0019172432
SN - 0300-8207
VL - 8
SP - 1
EP - 8
JO - Connective tissue research
JF - Connective tissue research
IS - 1
ER -