TY - JOUR
T1 - Skeletal muscle glucocorticoid receptor and glutamine synthetase activity in the wasting syndrome in rats treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin
AU - Max, Stephen R.
AU - Silbergeld, Ellen K.
N1 - Funding Information:
We thank Drs. G. Dmytrenko and B. H. Sohmer for helpful suggestions, Ms. M. A. Wennes for technical assistance, Ms. B. Pasko for preparation of the typescript. Drs. George Lucier and J. McKinney for the gift of TCDD, Dr. Thomas Gasiewicz for providing the safety plan, and Dr. Nelson Gamett for facilitating work in the Biohazard Facility. This work was supported in part by the Veterans Administration and by grants from NASA (NAG 2-loo), NIH (HD 16596), and the Bressler Fund and the Chancellor’s Fund ofthe University of Maryland School of Medicine.
PY - 1987/3/15
Y1 - 1987/3/15
N2 - This study demonstrated specific changes in rat skeletal muscles after a single oral dose (100 μg/kg) of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). The development of the wasting syndrome was characterized by marked body weight loss, as well as atrophy of plantaris and gastrocnemius muscles. Fourteen days after administration of TCDD, gastrocnemius muscle cytosolic glucocorticoid receptor binding, measured at a single saturating concentration of [3H]triamcinolone acetonide, was significantly diminished, while plantaris muscle glutamine synthetase activity was strikingly elevated, indicating that specific biochemical alterations occur in skeletal muscle in the wasting syndrome.
AB - This study demonstrated specific changes in rat skeletal muscles after a single oral dose (100 μg/kg) of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). The development of the wasting syndrome was characterized by marked body weight loss, as well as atrophy of plantaris and gastrocnemius muscles. Fourteen days after administration of TCDD, gastrocnemius muscle cytosolic glucocorticoid receptor binding, measured at a single saturating concentration of [3H]triamcinolone acetonide, was significantly diminished, while plantaris muscle glutamine synthetase activity was strikingly elevated, indicating that specific biochemical alterations occur in skeletal muscle in the wasting syndrome.
UR - http://www.scopus.com/inward/record.url?scp=0023090972&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023090972&partnerID=8YFLogxK
U2 - 10.1016/0041-008X(87)90258-4
DO - 10.1016/0041-008X(87)90258-4
M3 - Article
C2 - 2882621
AN - SCOPUS:0023090972
SN - 0041-008X
VL - 87
SP - 523
EP - 527
JO - Toxicology and Applied Pharmacology
JF - Toxicology and Applied Pharmacology
IS - 3
ER -