SIVQ-LCM protocol for the ArcturusXT instrument

Jason D. Hipp; Jerome Cheng; Jeffrey C. Hanson; Avi Z. Rosenberg; Michael R. Emmert-Buck; Michael A. Tangrea; Ulysses J. Balis

doi:10.3791/51662

SIVQ-LCM protocol for the ArcturusXT instrument

Jason D. Hipp, Jerome Cheng, Jeffrey C. Hanson, Avi Z. Rosenberg, Michael R. Emmert-Buck, Michael A. Tangrea, Ulysses J. Balis

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

SIVQ-LCM is a new methodology that automates and streamlines the more traditional, user-dependent laser dissection process. It aims to create an advanced, rapidly customizable laser dissection platform technology. In this report, we describe the integration of the image analysis software Spatially Invariant Vector Quantization (SIVQ) onto the ArcturusXT instrument. The ArcturusXT system contains both an infrared (IR) and ultraviolet (UV) laser, allowing for specific cell or large area dissections. The principal goal is to improve the speed, accuracy, and reproducibility of the laser dissection to increase sample throughput. This novel approach facilitates microdissection of both animal and human tissues in research and clinical workflows.

Original language	English (US)
Article number	e51662
Journal	Journal of Visualized Experiments
Issue number	89
DOIs	https://doi.org/10.3791/51662
State	Published - Jul 23 2014
Externally published	Yes

Keywords

ArcturusXT
Bioengineering
Digital pathology
Image analysis
Issue 89
LCM
Personalized medicine
SIVQ

ASJC Scopus subject areas

General Neuroscience
General Chemical Engineering
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology

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10.3791/51662

Cite this

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title = "SIVQ-LCM protocol for the ArcturusXT instrument",

abstract = "SIVQ-LCM is a new methodology that automates and streamlines the more traditional, user-dependent laser dissection process. It aims to create an advanced, rapidly customizable laser dissection platform technology. In this report, we describe the integration of the image analysis software Spatially Invariant Vector Quantization (SIVQ) onto the ArcturusXT instrument. The ArcturusXT system contains both an infrared (IR) and ultraviolet (UV) laser, allowing for specific cell or large area dissections. The principal goal is to improve the speed, accuracy, and reproducibility of the laser dissection to increase sample throughput. This novel approach facilitates microdissection of both animal and human tissues in research and clinical workflows.",

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doi = "10.3791/51662",

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AU - Hipp, Jason D.

AU - Cheng, Jerome

AU - Hanson, Jeffrey C.

AU - Rosenberg, Avi Z.

AU - Emmert-Buck, Michael R.

AU - Tangrea, Michael A.

AU - Balis, Ulysses J.

PY - 2014/7/23

Y1 - 2014/7/23

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SIVQ-LCM protocol for the ArcturusXT instrument

Abstract

Keywords

ASJC Scopus subject areas

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