Site-specific radiolabeling of monoclonal antibodies with biotin/streptavidin

Renato B. Del Rosario; Richard L. Wahl

doi:10.1016/0883-2897(89)90067-6

Site-specific radiolabeling of monoclonal antibodies with biotin/streptavidin

Renato B. Del Rosario
, Richard L. Wahl

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Sulfhydryl groups of a murine IgG2a monoclonal antibody, 5G6.4, were site-specifically biotinylated with N-iodo-acetyl-N′-biotinylhexylenediamine (compound 1) following partial reduction of disulfide bonds. Antibody labeling using fluoresceinated or [¹²⁵I]streptavidin were consistent with sulfhydryl group-specific incorporation of label with good retention of immunoreactivity, mainly in the form of high molecular weight oligomers (molecular weight ≈ > 400,000). In vivo localization of the radioantibody complex to xenografts of ovarian carcinoma was achieved. This approach appears to be a promising method of labeling monoclonal antibodies.

Original language	English (US)
Pages (from-to)	525-527,529
Journal	International journal of radiation applications and instrumentation. Part B, Nuclear medicine and biology
Volume	16
Issue number	5
DOIs	https://doi.org/10.1016/0883-2897(89)90067-6
State	Published - 1989
Externally published	Yes

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General Medicine

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title = "Site-specific radiolabeling of monoclonal antibodies with biotin/streptavidin",

abstract = "Sulfhydryl groups of a murine IgG2a monoclonal antibody, 5G6.4, were site-specifically biotinylated with N-iodo-acetyl-N′-biotinylhexylenediamine (compound 1) following partial reduction of disulfide bonds. Antibody labeling using fluoresceinated or [125I]streptavidin were consistent with sulfhydryl group-specific incorporation of label with good retention of immunoreactivity, mainly in the form of high molecular weight oligomers (molecular weight ≈ > 400,000). In vivo localization of the radioantibody complex to xenografts of ovarian carcinoma was achieved. This approach appears to be a promising method of labeling monoclonal antibodies.",

author = "\{Del Rosario\}, \{Renato B.\} and Wahl, \{Richard L.\}",

year = "1989",

doi = "10.1016/0883-2897(89)90067-6",

language = "English (US)",

volume = "16",

pages = "525--527,529",

journal = "International journal of radiation applications and instrumentation. Part B, Nuclear medicine and biology",

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publisher = "Elsevier BV",

number = "5",

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T1 - Site-specific radiolabeling of monoclonal antibodies with biotin/streptavidin

AU - Del Rosario, Renato B.

AU - Wahl, Richard L.

PY - 1989

Y1 - 1989

N2 - Sulfhydryl groups of a murine IgG2a monoclonal antibody, 5G6.4, were site-specifically biotinylated with N-iodo-acetyl-N′-biotinylhexylenediamine (compound 1) following partial reduction of disulfide bonds. Antibody labeling using fluoresceinated or [125I]streptavidin were consistent with sulfhydryl group-specific incorporation of label with good retention of immunoreactivity, mainly in the form of high molecular weight oligomers (molecular weight ≈ > 400,000). In vivo localization of the radioantibody complex to xenografts of ovarian carcinoma was achieved. This approach appears to be a promising method of labeling monoclonal antibodies.

AB - Sulfhydryl groups of a murine IgG2a monoclonal antibody, 5G6.4, were site-specifically biotinylated with N-iodo-acetyl-N′-biotinylhexylenediamine (compound 1) following partial reduction of disulfide bonds. Antibody labeling using fluoresceinated or [125I]streptavidin were consistent with sulfhydryl group-specific incorporation of label with good retention of immunoreactivity, mainly in the form of high molecular weight oligomers (molecular weight ≈ > 400,000). In vivo localization of the radioantibody complex to xenografts of ovarian carcinoma was achieved. This approach appears to be a promising method of labeling monoclonal antibodies.

UR - https://www.scopus.com/pages/publications/45249129898

UR - https://www.scopus.com/pages/publications/45249129898#tab=citedBy

U2 - 10.1016/0883-2897(89)90067-6

DO - 10.1016/0883-2897(89)90067-6

M3 - Article

C2 - 2807958

AN - SCOPUS:45249129898

SN - 0883-2897

VL - 16

SP - 525-527,529

JO - International journal of radiation applications and instrumentation. Part B, Nuclear medicine and biology

JF - International journal of radiation applications and instrumentation. Part B, Nuclear medicine and biology

IS - 5

ER -

Site-specific radiolabeling of monoclonal antibodies with biotin/streptavidin

Abstract

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