Single-tube multiplex-PCR screen for common deletional determinants of α-thalassemia

Samuel S. Chong; Corinne Dee Boehm; Douglas R. Higgs; Garry R. Cutting

doi:10.1182/blood.v95.1.360

Single-tube multiplex-PCR screen for common deletional determinants of α-thalassemia

Samuel S. Chong, Corinne Dee Boehm, Douglas R. Higgs, Garry R. Cutting

School of Medicine

Research output: Contribution to journal › Article › peer-review

395 Scopus citations

Abstract

α-Thalassemia is very common throughout all tropical and subtropical regions of the world. In Southeast Asia and the Mediterranean regions, compound heterozygotes and homozygotes may have anemia that is mild to severe (hemoglobin [Hb] H disease) or lethal (Hb Bart's hydrops fetalls). We have developed a reliable, single-tube multiplex-polymerase chain reaction (PCR) assay for the 6 most frequently observed determinants of α-thalassemia. The assay allows simple, high throughput genetic screening for these common hematological disorders.

Original language	English (US)
Pages (from-to)	360-362
Number of pages	3
Journal	Blood
Volume	95
Issue number	1
DOIs	https://doi.org/10.1182/blood.v95.1.360
State	Published - Jan 1 2000

ASJC Scopus subject areas

Biochemistry
Immunology
Hematology
Cell Biology

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AU - Higgs, Douglas R.

AU - Cutting, Garry R.

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AB - α-Thalassemia is very common throughout all tropical and subtropical regions of the world. In Southeast Asia and the Mediterranean regions, compound heterozygotes and homozygotes may have anemia that is mild to severe (hemoglobin [Hb] H disease) or lethal (Hb Bart's hydrops fetalls). We have developed a reliable, single-tube multiplex-polymerase chain reaction (PCR) assay for the 6 most frequently observed determinants of α-thalassemia. The assay allows simple, high throughput genetic screening for these common hematological disorders.

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Single-tube multiplex-PCR screen for common deletional determinants of α-thalassemia

Abstract

ASJC Scopus subject areas

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