Single-tube multiplex-PCR screen for common deletional determinants of α-thalassemia

Samuel S. Chong, Corinne Dee Boehm, Douglas R. Higgs, Garry R. Cutting

Research output: Contribution to journalArticlepeer-review

395 Scopus citations


α-Thalassemia is very common throughout all tropical and subtropical regions of the world. In Southeast Asia and the Mediterranean regions, compound heterozygotes and homozygotes may have anemia that is mild to severe (hemoglobin [Hb] H disease) or lethal (Hb Bart's hydrops fetalls). We have developed a reliable, single-tube multiplex-polymerase chain reaction (PCR) assay for the 6 most frequently observed determinants of α-thalassemia. The assay allows simple, high throughput genetic screening for these common hematological disorders.

Original languageEnglish (US)
Pages (from-to)360-362
Number of pages3
Issue number1
StatePublished - Jan 1 2000

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology


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