@inproceedings{3db0c542d1884c16ac77cbe646386b2b,
title = "Single quantum dot-based multiplexed point mutation detection by gap ligase chain reaction",
abstract = "We propose an assay which combines the specificity of gap-filling ligation chain reaction (Gap-LCR) and the sensitivity of quantum dot-single molecule spectroscopy (QD-SMS) to detect point mutation from genomic DNA without pre-PCR amplification. Mutation variant-specific ligation products are generated by Gap-LCR and captured by quantum dot to form DNA-QD nanocomplexes that are detected by SMS. SMS enables measurement of fluorescent bursts emitted from individual molecules, eliminating the need of separation of ligation products. The proposed assay is capable of detecting zeptomoles of KRAS codon 12 mutation variants and allows for detection of multiple types of mutation variants from a single experiment.",
keywords = "Gap ligase chain reaction, Mutation detection, Quantum dot, Single molecule spectroscopy",
author = "Yunke Song and Yi Zhang and Wang, \{Tza Huei\}",
year = "2011",
language = "English (US)",
isbn = "9781618395955",
series = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011",
pages = "1779--1781",
booktitle = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011",
note = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 ; Conference date: 02-10-2011 Through 06-10-2011",
}