TY - CHAP
T1 - Single-particle cryo-electron microscopy (cryo-EM)
T2 - Progress, challenges, and perspectives for further improvement
AU - Agard, David
AU - Cheng, Yifan
AU - Glaeser, Robert M.
AU - Subramaniam, Sriram
PY - 2014
Y1 - 2014
N2 - Electron microscopy (EM) of biological macromolecules has experienced a significant increase in its capabilities with the introduction of a new generation of camera technology. Near-atomic resolution now has been achieved without the need for ordered assemblies such as two-dimensional (2-D) crystals, helical structures, or icosahedral particles. Not only has the resolution achieved in single-particle cryo-electron microscopy (cryo-EM) improved markedly, but at the same time, the number of particles needed has been reduced by a factor of 10 or more. However, not all single-particle analyses may reach atomic resolution easily, and the reasons for this are diverse. Here, we address some of the factors that could result in lower-than-expected values of resolution, and we suggest strategies to identify and fix the possible problems.
AB - Electron microscopy (EM) of biological macromolecules has experienced a significant increase in its capabilities with the introduction of a new generation of camera technology. Near-atomic resolution now has been achieved without the need for ordered assemblies such as two-dimensional (2-D) crystals, helical structures, or icosahedral particles. Not only has the resolution achieved in single-particle cryo-electron microscopy (cryo-EM) improved markedly, but at the same time, the number of particles needed has been reduced by a factor of 10 or more. However, not all single-particle analyses may reach atomic resolution easily, and the reasons for this are diverse. Here, we address some of the factors that could result in lower-than-expected values of resolution, and we suggest strategies to identify and fix the possible problems.
KW - Biological specimens
KW - Resolution limitations
KW - Single-particle cryo-EM
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U2 - 10.1016/B978-0-12-800144-8.00002-1
DO - 10.1016/B978-0-12-800144-8.00002-1
M3 - Chapter
AN - SCOPUS:84905217139
SN - 9780128001448
VL - 185
T3 - Advances in Imaging and Electron Physics
SP - 113
EP - 137
BT - Advances in Imaging and Electron Physics
PB - Academic Press Inc.
ER -