Single-molecule fluorescence microscopy of native macromolecular complexes

Vasudha Aggarwal, Taekjip Ha

Research output: Contribution to journalReview articlepeer-review

25 Scopus citations


Macromolecular complexes consisting of proteins, lipids, and/or nucleic acids are ubiquitous in biological processes. Their composition, stoichiometry, order of assembly, and conformations can be heterogeneous or can change dynamically, making single-molecule studies best suited to measure these properties accurately. Recent single-molecule pull-down and other related approaches have combined the principles of conventional co-immunoprecipitation assay with single-molecule fluorescence microscopy to probe native macromolecular complexes. In this review, we present the advances in single-molecule pull-down methods and biological systems that have been investigated in such semi vivo manner.

Original languageEnglish (US)
Pages (from-to)225-232
Number of pages8
JournalCurrent Opinion in Structural Biology
StatePublished - Dec 1 2016

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology


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