Single-molecule fluorescence methods for the study of nucleic acids

Research output: Contribution to journalReview articlepeer-review

115 Scopus citations


Single-molecule fluorescence methods and biomechanical tools provide exciting new opportunities to probe biochemical processes in unprecedented detail. The detection and spectroscopy of single fluorophores have recently been used to observe conformational changes and biochemical events involving nucleic acids. A number of fluorescence observables, including localization, quenching, polarization response and fluorescence resonance energy transfer, have been utilized. An exciting new opportunity of combining fluorescence methods and biomechanical tools to study the structural changes and functions of enzymes that participate in nucleic acid metabolism has also arisen.

Original languageEnglish (US)
Pages (from-to)287-292
Number of pages6
JournalCurrent Opinion in Structural Biology
Issue number3
StatePublished - Jun 1 2001
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology


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