Single-molecule conformational analysis of G-quadruplex formation in the promoter DNA duplex of the proto-oncogene C-kit

Pravin S. Shirude; Burak Okumus; Liming Ying; Taekjip Ha; Shankar Balasubramanian

doi:10.1021/ja070497d

Single-molecule conformational analysis of G-quadruplex formation in the promoter DNA duplex of the proto-oncogene C-kit

Pravin S. Shirude, Burak Okumus, Liming Ying, Taekjip Ha, Shankar Balasubramanian

Research output: Contribution to journal › Article › peer-review

98 Scopus citations

Abstract

Herein we report on the single-molecule analysis of a conserved DNA quadruplex element found in the transcriptional activation site of the promoter of the proto- oncogene c-kit. We demonstrate that the c-kit quadruplex motif is able to fold into nonduplex states within a natural extended DNA duplex. There are apparent differences in putative quadruplex structures, and the K⁺ dependence on quadruplex formation, that appear to result when studying this quadruplex motif in natural duplex, as opposed to the single-stranded form.

Original language	English (US)
Pages (from-to)	7484-7485
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	129
Issue number	24
DOIs	https://doi.org/10.1021/ja070497d
State	Published - Jun 20 2007
Externally published	Yes

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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abstract = "Herein we report on the single-molecule analysis of a conserved DNA quadruplex element found in the transcriptional activation site of the promoter of the proto- oncogene c-kit. We demonstrate that the c-kit quadruplex motif is able to fold into nonduplex states within a natural extended DNA duplex. There are apparent differences in putative quadruplex structures, and the K+ dependence on quadruplex formation, that appear to result when studying this quadruplex motif in natural duplex, as opposed to the single-stranded form.",

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AU - Shirude, Pravin S.

AU - Okumus, Burak

AU - Ying, Liming

AU - Ha, Taekjip

AU - Balasubramanian, Shankar

PY - 2007/6/20

Y1 - 2007/6/20

N2 - Herein we report on the single-molecule analysis of a conserved DNA quadruplex element found in the transcriptional activation site of the promoter of the proto- oncogene c-kit. We demonstrate that the c-kit quadruplex motif is able to fold into nonduplex states within a natural extended DNA duplex. There are apparent differences in putative quadruplex structures, and the K+ dependence on quadruplex formation, that appear to result when studying this quadruplex motif in natural duplex, as opposed to the single-stranded form.

AB - Herein we report on the single-molecule analysis of a conserved DNA quadruplex element found in the transcriptional activation site of the promoter of the proto- oncogene c-kit. We demonstrate that the c-kit quadruplex motif is able to fold into nonduplex states within a natural extended DNA duplex. There are apparent differences in putative quadruplex structures, and the K+ dependence on quadruplex formation, that appear to result when studying this quadruplex motif in natural duplex, as opposed to the single-stranded form.

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Single-molecule conformational analysis of G-quadruplex formation in the promoter DNA duplex of the proto-oncogene C-kit

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