Simultaneous liquid chromatographic-electrospray ionization mass spectrometric quantification of 3,4-methylenedioxymethamphetamine (MDMA, Ecstasy) and its metabolites 3,4-dihydroxymethamphetamine, 4-hydroxy-3-methoxymethamphetamine and 3,4-methylenedioxyamphetamine in squirrel monkey and human plasma after acidic conjugate cleavage

Melanie Mueller; Frank T. Peters; Marilyn A. Huestis; George A. Ricaurte; Hans H. Maurer

doi:10.1016/j.forsciint.2008.12.002

Simultaneous liquid chromatographic-electrospray ionization mass spectrometric quantification of 3,4-methylenedioxymethamphetamine (MDMA, Ecstasy) and its metabolites 3,4-dihydroxymethamphetamine, 4-hydroxy-3-methoxymethamphetamine and 3,4-methylenedioxyamphetamine in squirrel monkey and human plasma after acidic conjugate cleavage

Melanie Mueller
, Frank T. Peters
, Marilyn A. Huestis
, George A. Ricaurte
, Hans H. Maurer

School of Medicine

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

3,4-Methylenedioxymethamphetamine (MDMA, Ecstasy) is a psychoactive drug with abuse liability and neurotoxic potential. Specimen preparation of a recently presented LC-MS assay with electrospray ionization for quantifying MDMA and its main metabolites in squirrel monkey plasma was modified to include acidic hydrolysis to obtain total 3,4-dihydroxymethamphetamine and 4-hydroxy-3-methoxy-methamphetamine. Method re-validation for squirrel monkey plasma and full validation for human plasma showed selectivity for all analytes. Recoveries were ≥71.0%. Changed specimen preparation or matrix did not affect accuracy or precision. No instability was observed after repeated freezing or in processed samples. Plasma MDMA and metabolites quantification, derived pharmacokinetic and toxicokinetic data and neurotoxicity research will benefit from this validated method.

Original language	English (US)
Pages (from-to)	64-68
Number of pages	5
Journal	Forensic Science International
Volume	184
Issue number	1-3
DOIs	https://doi.org/10.1016/j.forsciint.2008.12.002
State	Published - Jan 30 2009

Keywords

Acidic hydrolysis
Human plasma
LC-ESI-MS
MDMA
Metabolites
Validation

ASJC Scopus subject areas

Pathology and Forensic Medicine

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10.1016/j.forsciint.2008.12.002

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Mueller, M., Peters, F. T., Huestis, M. A., Ricaurte, G. A., & Maurer, H. H. (2009). Simultaneous liquid chromatographic-electrospray ionization mass spectrometric quantification of 3,4-methylenedioxymethamphetamine (MDMA, Ecstasy) and its metabolites 3,4-dihydroxymethamphetamine, 4-hydroxy-3-methoxymethamphetamine and 3,4-methylenedioxyamphetamine in squirrel monkey and human plasma after acidic conjugate cleavage. Forensic Science International, 184(1-3), 64-68. https://doi.org/10.1016/j.forsciint.2008.12.002

Simultaneous liquid chromatographic-electrospray ionization mass spectrometric quantification of 3,4-methylenedioxymethamphetamine (MDMA, Ecstasy) and its metabolites 3,4-dihydroxymethamphetamine, 4-hydroxy-3-methoxymethamphetamine and 3,4-methylenedioxyamphetamine in squirrel monkey and human plasma after acidic conjugate cleavage. / Mueller, Melanie; Peters, Frank T.; Huestis, Marilyn A. et al.
In: Forensic Science International, Vol. 184, No. 1-3, 30.01.2009, p. 64-68.

Research output: Contribution to journal › Article › peer-review

Mueller, M, Peters, FT, Huestis, MA, Ricaurte, GA & Maurer, HH 2009, 'Simultaneous liquid chromatographic-electrospray ionization mass spectrometric quantification of 3,4-methylenedioxymethamphetamine (MDMA, Ecstasy) and its metabolites 3,4-dihydroxymethamphetamine, 4-hydroxy-3-methoxymethamphetamine and 3,4-methylenedioxyamphetamine in squirrel monkey and human plasma after acidic conjugate cleavage', Forensic Science International, vol. 184, no. 1-3, pp. 64-68. https://doi.org/10.1016/j.forsciint.2008.12.002

Mueller M, Peters FT, Huestis MA, Ricaurte GA, Maurer HH. Simultaneous liquid chromatographic-electrospray ionization mass spectrometric quantification of 3,4-methylenedioxymethamphetamine (MDMA, Ecstasy) and its metabolites 3,4-dihydroxymethamphetamine, 4-hydroxy-3-methoxymethamphetamine and 3,4-methylenedioxyamphetamine in squirrel monkey and human plasma after acidic conjugate cleavage. Forensic Science International. 2009 Jan 30;184(1-3):64-68. doi: 10.1016/j.forsciint.2008.12.002

Mueller, Melanie ; Peters, Frank T. ; Huestis, Marilyn A. et al. / Simultaneous liquid chromatographic-electrospray ionization mass spectrometric quantification of 3,4-methylenedioxymethamphetamine (MDMA, Ecstasy) and its metabolites 3,4-dihydroxymethamphetamine, 4-hydroxy-3-methoxymethamphetamine and 3,4-methylenedioxyamphetamine in squirrel monkey and human plasma after acidic conjugate cleavage. In: Forensic Science International. 2009 ; Vol. 184, No. 1-3. pp. 64-68.

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abstract = "3,4-Methylenedioxymethamphetamine (MDMA, Ecstasy) is a psychoactive drug with abuse liability and neurotoxic potential. Specimen preparation of a recently presented LC-MS assay with electrospray ionization for quantifying MDMA and its main metabolites in squirrel monkey plasma was modified to include acidic hydrolysis to obtain total 3,4-dihydroxymethamphetamine and 4-hydroxy-3-methoxy-methamphetamine. Method re-validation for squirrel monkey plasma and full validation for human plasma showed selectivity for all analytes. Recoveries were ≥71.0\%. Changed specimen preparation or matrix did not affect accuracy or precision. No instability was observed after repeated freezing or in processed samples. Plasma MDMA and metabolites quantification, derived pharmacokinetic and toxicokinetic data and neurotoxicity research will benefit from this validated method.",

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