Simultaneous determination of trimethoprim and sulfamethoxazole in dried plasma and urine spots

Daniel Gonzalez; Chiara Melloni; Brenda B. Poindexter; Ram Yogev; Andrew M. Atz; Janice E. Sullivan; Susan R. Mendley; Paula Delmore; Amy Delinsky; Kanecia Zimmerman; Andrew Lewandowski; Barrie Harper; Kenneth C. Lewis; Daniel K. Benjamin; Michael Cohen-Wolkowiez; K. Y. Berezny; E. Capparelli; G. L. Kearns; M. Laughon; A. Muelenaer; T. M. O'Shea; I. M. Paul; P. B. Smith; J. Van Den Anker; K. Wade; T. J. Walsh; D. Siegel; P. Taylor-Zapata; A. Zajicek; Z. Ren; K. Tsilou; A. Pagan; R. Anand; G. Simone; A. Lewandowski; B. Harper; L. Smiley; R. Yogev; L. Fern; H. Al Nasiri; J. Sullivan; T. Bratton; Donna Cannonier

doi:10.4155/bio.15.38

Simultaneous determination of trimethoprim and sulfamethoxazole in dried plasma and urine spots

Daniel Gonzalez, Chiara Melloni, Brenda B. Poindexter, Ram Yogev, Andrew M. Atz, Janice E. Sullivan, Susan R. Mendley, Paula Delmore, Amy Delinsky, Kanecia Zimmerman, Andrew Lewandowski, Barrie Harper, Kenneth C. Lewis, Daniel K. Benjamin, Michael Cohen-Wolkowiez, K. Y. Berezny, E. Capparelli, G. L. Kearns, M. Laughon, A. MuelenaerT. M. O'Shea, I. M. Paul, P. B. Smith, J. Van Den Anker, K. Wade, T. J. Walsh, D. Siegel, P. Taylor-Zapata, A. Zajicek, Z. Ren, K. Tsilou, A. Pagan, R. Anand, G. Simone, A. Lewandowski, B. Harper, L. Smiley, R. Yogev, L. Fern, H. Al Nasiri, J. Sullivan, T. Bratton, Donna Cannonier

School of Medicine

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Background: Trimethoprim-sulfamethoxazole (TMP-SMX) is an antimicrobial drug combination commonly prescribed in children and adults. The study objectives were to validate and apply an HPLC-MS/MS method to quantify TMP-SMX in dried plasma spots (DPS) and dried urine spots (DUS), and perform a comparability analysis with liquid matrices. Results: For TMP the validated range was 100-50,000 ng/ml for DPS and 500-250,000 ng/ml for DUS; for SMX, the validated range was 1000-500,000 ng/ml for both DPS and DUS. Good agreement was noted between DPS/DUS and liquid plasma and urine samples for TMP, while only modest agreement was observed for SMX in both matrices. Conclusion: A precise, accurate and reproducible method was developed to quantify TMP-SMX in DPS and DUS samples.

Original language	English (US)
Pages (from-to)	1137-1149
Number of pages	13
Journal	Bioanalysis
Volume	7
Issue number	9
DOIs	https://doi.org/10.4155/bio.15.38
State	Published - May 1 2015

ASJC Scopus subject areas

Analytical Chemistry
General Pharmacology, Toxicology and Pharmaceutics
Clinical Biochemistry
Medical Laboratory Technology

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Gonzalez, D., Melloni, C., Poindexter, B. B., Yogev, R., Atz, A. M., Sullivan, J. E., Mendley, S. R., Delmore, P., Delinsky, A., Zimmerman, K., Lewandowski, A., Harper, B., Lewis, K. C., Benjamin, D. K., Cohen-Wolkowiez, M., Berezny, K. Y., Capparelli, E., Kearns, G. L., Laughon, M., ... Cannonier, D. (2015). Simultaneous determination of trimethoprim and sulfamethoxazole in dried plasma and urine spots. Bioanalysis, 7(9), 1137-1149. https://doi.org/10.4155/bio.15.38

Gonzalez, D, Melloni, C, Poindexter, BB, Yogev, R, Atz, AM, Sullivan, JE, Mendley, SR, Delmore, P, Delinsky, A, Zimmerman, K, Lewandowski, A, Harper, B, Lewis, KC, Benjamin, DK, Cohen-Wolkowiez, M, Berezny, KY, Capparelli, E, Kearns, GL, Laughon, M, Muelenaer, A, O'Shea, TM, Paul, IM, Smith, PB, Van Den Anker, J, Wade, K, Walsh, TJ, Siegel, D, Taylor-Zapata, P, Zajicek, A, Ren, Z, Tsilou, K, Pagan, A, Anand, R, Simone, G, Lewandowski, A, Harper, B, Smiley, L, Yogev, R, Fern, L, Al Nasiri, H, Sullivan, J, Bratton, T & Cannonier, D 2015, 'Simultaneous determination of trimethoprim and sulfamethoxazole in dried plasma and urine spots', Bioanalysis, vol. 7, no. 9, pp. 1137-1149. https://doi.org/10.4155/bio.15.38

@article{e4e2914404cb4377a4e9b91ef08d4c5f,

title = "Simultaneous determination of trimethoprim and sulfamethoxazole in dried plasma and urine spots",

abstract = "Background: Trimethoprim-sulfamethoxazole (TMP-SMX) is an antimicrobial drug combination commonly prescribed in children and adults. The study objectives were to validate and apply an HPLC-MS/MS method to quantify TMP-SMX in dried plasma spots (DPS) and dried urine spots (DUS), and perform a comparability analysis with liquid matrices. Results: For TMP the validated range was 100-50,000 ng/ml for DPS and 500-250,000 ng/ml for DUS; for SMX, the validated range was 1000-500,000 ng/ml for both DPS and DUS. Good agreement was noted between DPS/DUS and liquid plasma and urine samples for TMP, while only modest agreement was observed for SMX in both matrices. Conclusion: A precise, accurate and reproducible method was developed to quantify TMP-SMX in DPS and DUS samples.",

author = "Daniel Gonzalez and Chiara Melloni and Poindexter, {Brenda B.} and Ram Yogev and Atz, {Andrew M.} and Sullivan, {Janice E.} and Mendley, {Susan R.} and Paula Delmore and Amy Delinsky and Kanecia Zimmerman and Andrew Lewandowski and Barrie Harper and Lewis, {Kenneth C.} and Benjamin, {Daniel K.} and Michael Cohen-Wolkowiez and Berezny, {K. Y.} and E. Capparelli and Kearns, {G. L.} and M. Laughon and A. Muelenaer and O'Shea, {T. M.} and Paul, {I. M.} and Smith, {P. B.} and {Van Den Anker}, J. and K. Wade and Walsh, {T. J.} and D. Siegel and P. Taylor-Zapata and A. Zajicek and Z. Ren and K. Tsilou and A. Pagan and R. Anand and G. Simone and A. Lewandowski and B. Harper and L. Smiley and R. Yogev and L. Fern and {Al Nasiri}, H. and J. Sullivan and T. Bratton and Donna Cannonier",

note = "Publisher Copyright: {\textcopyright} 2015 Future Science Ltd.",

year = "2015",

month = may,

day = "1",

doi = "10.4155/bio.15.38",

language = "English (US)",

volume = "7",

pages = "1137--1149",

journal = "Bioanalysis",

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TY - JOUR

T1 - Simultaneous determination of trimethoprim and sulfamethoxazole in dried plasma and urine spots

AU - Gonzalez, Daniel

AU - Melloni, Chiara

AU - Poindexter, Brenda B.

AU - Yogev, Ram

AU - Atz, Andrew M.

AU - Sullivan, Janice E.

AU - Mendley, Susan R.

AU - Delmore, Paula

AU - Delinsky, Amy

AU - Zimmerman, Kanecia

AU - Lewandowski, Andrew

AU - Harper, Barrie

AU - Lewis, Kenneth C.

AU - Benjamin, Daniel K.

AU - Cohen-Wolkowiez, Michael

AU - Berezny, K. Y.

AU - Capparelli, E.

AU - Kearns, G. L.

AU - Laughon, M.

AU - Muelenaer, A.

AU - O'Shea, T. M.

AU - Paul, I. M.

AU - Smith, P. B.

AU - Van Den Anker, J.

AU - Wade, K.

AU - Walsh, T. J.

AU - Siegel, D.

AU - Taylor-Zapata, P.

AU - Zajicek, A.

AU - Ren, Z.

AU - Tsilou, K.

AU - Pagan, A.

AU - Anand, R.

AU - Simone, G.

AU - Lewandowski, A.

AU - Harper, B.

AU - Smiley, L.

AU - Yogev, R.

AU - Fern, L.

AU - Al Nasiri, H.

AU - Sullivan, J.

AU - Bratton, T.

AU - Cannonier, Donna

PY - 2015/5/1

Y1 - 2015/5/1

N2 - Background: Trimethoprim-sulfamethoxazole (TMP-SMX) is an antimicrobial drug combination commonly prescribed in children and adults. The study objectives were to validate and apply an HPLC-MS/MS method to quantify TMP-SMX in dried plasma spots (DPS) and dried urine spots (DUS), and perform a comparability analysis with liquid matrices. Results: For TMP the validated range was 100-50,000 ng/ml for DPS and 500-250,000 ng/ml for DUS; for SMX, the validated range was 1000-500,000 ng/ml for both DPS and DUS. Good agreement was noted between DPS/DUS and liquid plasma and urine samples for TMP, while only modest agreement was observed for SMX in both matrices. Conclusion: A precise, accurate and reproducible method was developed to quantify TMP-SMX in DPS and DUS samples.

AB - Background: Trimethoprim-sulfamethoxazole (TMP-SMX) is an antimicrobial drug combination commonly prescribed in children and adults. The study objectives were to validate and apply an HPLC-MS/MS method to quantify TMP-SMX in dried plasma spots (DPS) and dried urine spots (DUS), and perform a comparability analysis with liquid matrices. Results: For TMP the validated range was 100-50,000 ng/ml for DPS and 500-250,000 ng/ml for DUS; for SMX, the validated range was 1000-500,000 ng/ml for both DPS and DUS. Good agreement was noted between DPS/DUS and liquid plasma and urine samples for TMP, while only modest agreement was observed for SMX in both matrices. Conclusion: A precise, accurate and reproducible method was developed to quantify TMP-SMX in DPS and DUS samples.

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SN - 1757-6180

VL - 7

SP - 1137

EP - 1149

JO - Bioanalysis

JF - Bioanalysis

IS - 9

ER -

Simultaneous determination of trimethoprim and sulfamethoxazole in dried plasma and urine spots

Abstract

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