Simultaneous determination of trimethoprim and sulfamethoxazole in dried plasma and urine spots

Daniel Gonzalez, Chiara Melloni, Brenda B. Poindexter, Ram Yogev, Andrew M. Atz, Janice E. Sullivan, Susan R. Mendley, Paula Delmore, Amy Delinsky, Kanecia Zimmerman, Andrew Lewandowski, Barrie Harper, Kenneth C. Lewis, Daniel K. Benjamin, Michael Cohen-Wolkowiez, K. Y. Berezny, E. Capparelli, G. L. Kearns, M. Laughon, A. MuelenaerT. M. O'Shea, I. M. Paul, P. B. Smith, J. Van Den Anker, K. Wade, T. J. Walsh, D. Siegel, P. Taylor-Zapata, A. Zajicek, Z. Ren, K. Tsilou, A. Pagan, R. Anand, G. Simone, A. Lewandowski, B. Harper, L. Smiley, R. Yogev, L. Fern, H. Al Nasiri, J. Sullivan, T. Bratton, Donna Cannonier

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


Background: Trimethoprim-sulfamethoxazole (TMP-SMX) is an antimicrobial drug combination commonly prescribed in children and adults. The study objectives were to validate and apply an HPLC-MS/MS method to quantify TMP-SMX in dried plasma spots (DPS) and dried urine spots (DUS), and perform a comparability analysis with liquid matrices. Results: For TMP the validated range was 100-50,000 ng/ml for DPS and 500-250,000 ng/ml for DUS; for SMX, the validated range was 1000-500,000 ng/ml for both DPS and DUS. Good agreement was noted between DPS/DUS and liquid plasma and urine samples for TMP, while only modest agreement was observed for SMX in both matrices. Conclusion: A precise, accurate and reproducible method was developed to quantify TMP-SMX in DPS and DUS samples.

Original languageEnglish (US)
Pages (from-to)1137-1149
Number of pages13
Issue number9
StatePublished - May 1 2015

ASJC Scopus subject areas

  • Analytical Chemistry
  • General Pharmacology, Toxicology and Pharmaceutics
  • Medical Laboratory Technology
  • Clinical Biochemistry


Dive into the research topics of 'Simultaneous determination of trimethoprim and sulfamethoxazole in dried plasma and urine spots'. Together they form a unique fingerprint.

Cite this