Silver cholinesterase immunocytochemistry: A new neuromuscular junction stain

Kathy Alderson; Alan Pestronk; Woon‐Che ‐C Yee; Daniel B. Drachman

doi:10.1002/mus.880120103

Silver cholinesterase immunocytochemistry: A new neuromuscular junction stain

Kathy Alderson, Alan Pestronk, Woon‐Che ‐C Yee, Daniel B. Drachman

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Evaluation of morphological alterations at the neuromuscular junction associated with sprouting or other pathological changes has been limited by the inability to visualize simultaneously the multiple cell types that make up a junction. A new combined stain for the concurrent demonstration of motor nerve terminals, cholinesterase, and Schwann cell myelin and other antigens at neuromuscular junctions using bromoindoxyl acetate dye staining for cholinesterase, silver‐gold impregnation for nerve terminals, and immunocytochemistry of selected antigens is described. The clarity of the stain permits graphic demonstration of the alteration of neuromuscular junction components during sprouting as well as other pathological changes.

Original language	English (US)
Pages (from-to)	9-14
Number of pages	6
Journal	Muscle & nerve
Volume	12
Issue number	1
DOIs	https://doi.org/10.1002/mus.880120103
State	Published - Jan 1989
Externally published	Yes

Keywords

amyotrophic lateral sclerosis
botulinum toxin
immunocytochemistry
neurofilaments
neuromuscular junction
peripheral neuropathy
silver stain

ASJC Scopus subject areas

Physiology
Clinical Neurology
Cellular and Molecular Neuroscience
Physiology (medical)

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10.1002/mus.880120103

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title = "Silver cholinesterase immunocytochemistry: A new neuromuscular junction stain",

abstract = "Evaluation of morphological alterations at the neuromuscular junction associated with sprouting or other pathological changes has been limited by the inability to visualize simultaneously the multiple cell types that make up a junction. A new combined stain for the concurrent demonstration of motor nerve terminals, cholinesterase, and Schwann cell myelin and other antigens at neuromuscular junctions using bromoindoxyl acetate dye staining for cholinesterase, silver‐gold impregnation for nerve terminals, and immunocytochemistry of selected antigens is described. The clarity of the stain permits graphic demonstration of the alteration of neuromuscular junction components during sprouting as well as other pathological changes.",

keywords = "amyotrophic lateral sclerosis, botulinum toxin, immunocytochemistry, neurofilaments, neuromuscular junction, peripheral neuropathy, silver stain",

author = "Kathy Alderson and Alan Pestronk and Yee, {Woon‐Che ‐C} and Drachman, {Daniel B.}",

year = "1989",

month = jan,

doi = "10.1002/mus.880120103",

language = "English (US)",

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T1 - Silver cholinesterase immunocytochemistry

T2 - A new neuromuscular junction stain

AU - Alderson, Kathy

AU - Pestronk, Alan

AU - Yee, Woon‐Che ‐C

AU - Drachman, Daniel B.

PY - 1989/1

Y1 - 1989/1

N2 - Evaluation of morphological alterations at the neuromuscular junction associated with sprouting or other pathological changes has been limited by the inability to visualize simultaneously the multiple cell types that make up a junction. A new combined stain for the concurrent demonstration of motor nerve terminals, cholinesterase, and Schwann cell myelin and other antigens at neuromuscular junctions using bromoindoxyl acetate dye staining for cholinesterase, silver‐gold impregnation for nerve terminals, and immunocytochemistry of selected antigens is described. The clarity of the stain permits graphic demonstration of the alteration of neuromuscular junction components during sprouting as well as other pathological changes.

AB - Evaluation of morphological alterations at the neuromuscular junction associated with sprouting or other pathological changes has been limited by the inability to visualize simultaneously the multiple cell types that make up a junction. A new combined stain for the concurrent demonstration of motor nerve terminals, cholinesterase, and Schwann cell myelin and other antigens at neuromuscular junctions using bromoindoxyl acetate dye staining for cholinesterase, silver‐gold impregnation for nerve terminals, and immunocytochemistry of selected antigens is described. The clarity of the stain permits graphic demonstration of the alteration of neuromuscular junction components during sprouting as well as other pathological changes.

KW - amyotrophic lateral sclerosis

KW - botulinum toxin

KW - immunocytochemistry

KW - neurofilaments

KW - neuromuscular junction

KW - peripheral neuropathy

KW - silver stain

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Silver cholinesterase immunocytochemistry: A new neuromuscular junction stain

Abstract

Keywords

ASJC Scopus subject areas

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