Signal-to-noise ratio analysis and improvement for fluorescence tomography imaging

Huiquan Wang; Xing Feng; Boya Shi; Wenxuan Liang; Yongping Chen; Jinhai Wang; Xingde Li

doi:10.1063/1.5045511

Signal-to-noise ratio analysis and improvement for fluorescence tomography imaging

Huiquan Wang, Xing Feng, Boya Shi, Wenxuan Liang, Yongping Chen, Jinhai Wang, Xingde Li

School of Medicine

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

CCD-based fluorescence tomography is widely used for small animal whole-body imaging. In this report, systematic signal-to-noise ratio (SNR) analyses of a fluorescence tomography imaging (FTI) system were performed, resulting in an easy-to-follow strategy to optimize hardware configurations and operational conditions for acquiring high-quality imaging data and for improving the overall system performance. Phantom experiments were conducted to demonstrate the performance improvement by these optimizations. The improved performance was further verified by imaging a tumor-bearing mouse in vivo. This report provides general and practical guidelines for setting up a high-performance electron multiplying charge coupled device based FTI system to achieve an optimized SNR, which can be useful for future FTI technology development.

Original language	English (US)
Article number	093114
Journal	Review of Scientific Instruments
Volume	89
Issue number	9
DOIs	https://doi.org/10.1063/1.5045511
State	Published - Sep 1 2018

ASJC Scopus subject areas

Instrumentation

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10.1063/1.5045511

Cite this

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title = "Signal-to-noise ratio analysis and improvement for fluorescence tomography imaging",

abstract = "CCD-based fluorescence tomography is widely used for small animal whole-body imaging. In this report, systematic signal-to-noise ratio (SNR) analyses of a fluorescence tomography imaging (FTI) system were performed, resulting in an easy-to-follow strategy to optimize hardware configurations and operational conditions for acquiring high-quality imaging data and for improving the overall system performance. Phantom experiments were conducted to demonstrate the performance improvement by these optimizations. The improved performance was further verified by imaging a tumor-bearing mouse in vivo. This report provides general and practical guidelines for setting up a high-performance electron multiplying charge coupled device based FTI system to achieve an optimized SNR, which can be useful for future FTI technology development.",

author = "Huiquan Wang and Xing Feng and Boya Shi and Wenxuan Liang and Yongping Chen and Jinhai Wang and Xingde Li",

note = "Funding Information: The authors would like to acknowledge useful discussions with Dr. Jiefeng Xi in the Department of Biomedical Engineering Johns Hopkins University. This work was supported in part by funding from the National Institutes of Health under Grant No. R01CA120480 and the China Postdoctoral Science Foundation of the Sixty-first Batch of Funds. Publisher Copyright: {\textcopyright} 2018 Author(s).",

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doi = "10.1063/1.5045511",

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T1 - Signal-to-noise ratio analysis and improvement for fluorescence tomography imaging

AU - Wang, Huiquan

AU - Feng, Xing

AU - Shi, Boya

AU - Liang, Wenxuan

AU - Chen, Yongping

AU - Wang, Jinhai

AU - Li, Xingde

N1 - Funding Information: The authors would like to acknowledge useful discussions with Dr. Jiefeng Xi in the Department of Biomedical Engineering Johns Hopkins University. This work was supported in part by funding from the National Institutes of Health under Grant No. R01CA120480 and the China Postdoctoral Science Foundation of the Sixty-first Batch of Funds. Publisher Copyright: © 2018 Author(s).

PY - 2018/9/1

Y1 - 2018/9/1

N2 - CCD-based fluorescence tomography is widely used for small animal whole-body imaging. In this report, systematic signal-to-noise ratio (SNR) analyses of a fluorescence tomography imaging (FTI) system were performed, resulting in an easy-to-follow strategy to optimize hardware configurations and operational conditions for acquiring high-quality imaging data and for improving the overall system performance. Phantom experiments were conducted to demonstrate the performance improvement by these optimizations. The improved performance was further verified by imaging a tumor-bearing mouse in vivo. This report provides general and practical guidelines for setting up a high-performance electron multiplying charge coupled device based FTI system to achieve an optimized SNR, which can be useful for future FTI technology development.

AB - CCD-based fluorescence tomography is widely used for small animal whole-body imaging. In this report, systematic signal-to-noise ratio (SNR) analyses of a fluorescence tomography imaging (FTI) system were performed, resulting in an easy-to-follow strategy to optimize hardware configurations and operational conditions for acquiring high-quality imaging data and for improving the overall system performance. Phantom experiments were conducted to demonstrate the performance improvement by these optimizations. The improved performance was further verified by imaging a tumor-bearing mouse in vivo. This report provides general and practical guidelines for setting up a high-performance electron multiplying charge coupled device based FTI system to achieve an optimized SNR, which can be useful for future FTI technology development.

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Signal-to-noise ratio analysis and improvement for fluorescence tomography imaging

Abstract

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