Shining a New Light on RNA-Protein Interactions

Jeffry L. Corden

doi:10.1016/j.chembiol.2010.04.003

Shining a New Light on RNA-Protein Interactions

Jeffry L. Corden

School of Medicine

Research output: Contribution to journal › Short survey › peer-review

2 Scopus citations

Abstract

PAR-CLIP is a new technique for transcriptome-wide mapping of binding sites for RNA-binding proteins. In this technique, a photoactivatable nucleoside is incorporated into RNA and crosslinked to proteins in vivo with UV light. The crosslinked nucleoside causes a specific base change during processing for deep sequencing, allowing the precise identification of binding sites.

Original language	English (US)
Pages (from-to)	316-318
Number of pages	3
Journal	Chemistry and Biology
Volume	17
Issue number	4
DOIs	https://doi.org/10.1016/j.chembiol.2010.04.003
State	Published - Apr 23 2010

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmacology
Drug Discovery
Clinical Biochemistry

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10.1016/j.chembiol.2010.04.003

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title = "Shining a New Light on RNA-Protein Interactions",

abstract = "PAR-CLIP is a new technique for transcriptome-wide mapping of binding sites for RNA-binding proteins. In this technique, a photoactivatable nucleoside is incorporated into RNA and crosslinked to proteins in vivo with UV light. The crosslinked nucleoside causes a specific base change during processing for deep sequencing, allowing the precise identification of binding sites.",

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doi = "10.1016/j.chembiol.2010.04.003",

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T1 - Shining a New Light on RNA-Protein Interactions

AU - Corden, Jeffry L.

PY - 2010/4/23

Y1 - 2010/4/23

N2 - PAR-CLIP is a new technique for transcriptome-wide mapping of binding sites for RNA-binding proteins. In this technique, a photoactivatable nucleoside is incorporated into RNA and crosslinked to proteins in vivo with UV light. The crosslinked nucleoside causes a specific base change during processing for deep sequencing, allowing the precise identification of binding sites.

AB - PAR-CLIP is a new technique for transcriptome-wide mapping of binding sites for RNA-binding proteins. In this technique, a photoactivatable nucleoside is incorporated into RNA and crosslinked to proteins in vivo with UV light. The crosslinked nucleoside causes a specific base change during processing for deep sequencing, allowing the precise identification of binding sites.

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U2 - 10.1016/j.chembiol.2010.04.003

DO - 10.1016/j.chembiol.2010.04.003

M3 - Short survey

C2 - 20416501

AN - SCOPUS:77950925558

SN - 1074-5521

VL - 17

SP - 316

EP - 318

JO - Chemistry and Biology

JF - Chemistry and Biology

IS - 4

ER -

Shining a New Light on RNA-Protein Interactions

Abstract

ASJC Scopus subject areas

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