TY - JOUR
T1 - Shiga toxin is transported into the nucleoli of intestinal epithelial cells via a carrier-dependent process
AU - Baibakov, Boris
AU - Murtazina, Rakhilya
AU - Elowsky, Christian
AU - Giardiello, Francis M.
AU - Kovbasnjuk, Olga
PY - 2010/6
Y1 - 2010/6
N2 - Shiga toxin (Stx) produced by the invasive Shigella dysenteriae serotype 1 (S. dysenteriae1) causes gastrointestinal and kidney complications. It has been assumed that Stx is released intracellularly after enterocyte invasion by S. dysenteriae1. However, there is little information about Stx distribution inside S. dysenteriae1-infected enterocytes. Here, we use intestinal epithelial T84 cells to characterize the trafficking of Stx delivered into the cytosol, in ways that mimic aspects of S. dysenteriae1 infection. We find that cytoplasmic Stx is transported into nucleoli. Stx nucleolar movement is carrier- and energy-dependent. Stx binding to the nucleoli of normal human enterocytes in vitro supports possible roles for nucleolar trafficking in toxin-induced intestinal pathology. 2010 by the authors; licensee MDPI, Basel, Switzerland.
AB - Shiga toxin (Stx) produced by the invasive Shigella dysenteriae serotype 1 (S. dysenteriae1) causes gastrointestinal and kidney complications. It has been assumed that Stx is released intracellularly after enterocyte invasion by S. dysenteriae1. However, there is little information about Stx distribution inside S. dysenteriae1-infected enterocytes. Here, we use intestinal epithelial T84 cells to characterize the trafficking of Stx delivered into the cytosol, in ways that mimic aspects of S. dysenteriae1 infection. We find that cytoplasmic Stx is transported into nucleoli. Stx nucleolar movement is carrier- and energy-dependent. Stx binding to the nucleoli of normal human enterocytes in vitro supports possible roles for nucleolar trafficking in toxin-induced intestinal pathology. 2010 by the authors; licensee MDPI, Basel, Switzerland.
KW - Intestinal epithelial cells
KW - Nucleolar trafficking
KW - Shiga toxin
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U2 - 10.3390/toxins2061318
DO - 10.3390/toxins2061318
M3 - Article
C2 - 22069640
AN - SCOPUS:79952082530
SN - 2072-6651
VL - 2
SP - 1318
EP - 1335
JO - Toxins
JF - Toxins
IS - 6
ER -