SFR3-DR5, a monoclonal antibody with HLA-DR5 specificity

S. F. Radka; C. Machamer; P. Cresswell; D. D. Kostyu; F. E. Ward; D. B. Amos

SFR3-DR5, a monoclonal antibody with HLA-DR5 specificity

S. F. Radka, C. Machamer, P. Cresswell, D. D. Kostyu, F. E. Ward, D. B. Amos

Research output: Contribution to journal › Article › peer-review

Abstract

We have developed a monoclonal antibody with HLA-DR5 serologic specificity. The antibody, SRF3-DR5, binds specifically to DR5-positive lymphoblastoid cell lines, and immunoprecipitates α- and β-chains characteristic of DR antigens from them. Cytotoxic activity of the antibody segregates with the DR5-bearing haplotype in a family. The antibody reacted with the cells of 16 of 17 DR5 individuals and was negative on all DR5-negative cells tested. SFR3-DR5 reacted weakly with PWM-activated cells of the single DR5 individual whose B lymphocytes were unreactive with the monoclonal antibody by cytotoxicity. Possible interpretations of these results are discussed.

Original language	English (US)
Pages (from-to)	1863-1866
Number of pages	4
Journal	Journal of Immunology
Volume	130
Issue number	4
State	Published - Jul 7 1983
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Immunology

Cite this

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title = "SFR3-DR5, a monoclonal antibody with HLA-DR5 specificity",

abstract = "We have developed a monoclonal antibody with HLA-DR5 serologic specificity. The antibody, SRF3-DR5, binds specifically to DR5-positive lymphoblastoid cell lines, and immunoprecipitates α- and β-chains characteristic of DR antigens from them. Cytotoxic activity of the antibody segregates with the DR5-bearing haplotype in a family. The antibody reacted with the cells of 16 of 17 DR5 individuals and was negative on all DR5-negative cells tested. SFR3-DR5 reacted weakly with PWM-activated cells of the single DR5 individual whose B lymphocytes were unreactive with the monoclonal antibody by cytotoxicity. Possible interpretations of these results are discussed.",

author = "Radka, {S. F.} and C. Machamer and P. Cresswell and Kostyu, {D. D.} and Ward, {F. E.} and Amos, {D. B.}",

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T1 - SFR3-DR5, a monoclonal antibody with HLA-DR5 specificity

AU - Radka, S. F.

AU - Machamer, C.

AU - Cresswell, P.

AU - Kostyu, D. D.

AU - Ward, F. E.

AU - Amos, D. B.

PY - 1983/7/7

Y1 - 1983/7/7

N2 - We have developed a monoclonal antibody with HLA-DR5 serologic specificity. The antibody, SRF3-DR5, binds specifically to DR5-positive lymphoblastoid cell lines, and immunoprecipitates α- and β-chains characteristic of DR antigens from them. Cytotoxic activity of the antibody segregates with the DR5-bearing haplotype in a family. The antibody reacted with the cells of 16 of 17 DR5 individuals and was negative on all DR5-negative cells tested. SFR3-DR5 reacted weakly with PWM-activated cells of the single DR5 individual whose B lymphocytes were unreactive with the monoclonal antibody by cytotoxicity. Possible interpretations of these results are discussed.

AB - We have developed a monoclonal antibody with HLA-DR5 serologic specificity. The antibody, SRF3-DR5, binds specifically to DR5-positive lymphoblastoid cell lines, and immunoprecipitates α- and β-chains characteristic of DR antigens from them. Cytotoxic activity of the antibody segregates with the DR5-bearing haplotype in a family. The antibody reacted with the cells of 16 of 17 DR5 individuals and was negative on all DR5-negative cells tested. SFR3-DR5 reacted weakly with PWM-activated cells of the single DR5 individual whose B lymphocytes were unreactive with the monoclonal antibody by cytotoxicity. Possible interpretations of these results are discussed.

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ER -

SFR3-DR5, a monoclonal antibody with HLA-DR5 specificity

Abstract

ASJC Scopus subject areas

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