Serum from interferon-β-1b-treated patients with early multiple sclerosis stabilizes the blood-brain barrier in vitro

Marcus Müller; Achim Frese; Isabelle Nassenstein; Maike Hoppen; Martin Marziniak; Erich Bernd Ringelstein; Kwang Sik Kim; Wolf Rüdiger Schäbitz; Jörg Kraus

doi:10.1177/1352458511416837

Serum from interferon-β-1b-treated patients with early multiple sclerosis stabilizes the blood-brain barrier in vitro

Marcus Müller, Achim Frese, Isabelle Nassenstein, Maike Hoppen, Martin Marziniak, Erich Bernd Ringelstein, Kwang Sik Kim, Wolf Rüdiger Schäbitz, Jörg Kraus

School of Medicine

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Interferon-β (IFN-β) stabilizes the blood-brain barrier (BBB) in vitro. Here we investigated the effect of serum from 15 IFN-β-1b-treated multiple sclerosis (MS) patients on the permeability read-outs of small solutes in an in vitro BBB model consisting of human brain microvascular endothelial cells in co-culture with rat astrocytes. The addition of sera from IFN-β-treated patients resulted in a significantly (p < 0.05) reduced permeability as compared with untreated patients. Our findings show that sera from IFN-β-1b-treated MS patients have a stabilizing effect on the in vitro BBB. We suggest an unknown potentially pro-inflammatory factor in the serum of MS patients that may lead to a BBB dysfunction and can be modulated by IFN-β.

Original language	English (US)
Pages (from-to)	236-239
Number of pages	4
Journal	Multiple Sclerosis Journal
Volume	18
Issue number	2
DOIs	https://doi.org/10.1177/1352458511416837
State	Published - Feb 2012

Keywords

bioassay
blood-brain barrier
interferon-β
multiple sclerosis

ASJC Scopus subject areas

Neurology
Clinical Neurology

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10.1177/1352458511416837

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title = "Serum from interferon-β-1b-treated patients with early multiple sclerosis stabilizes the blood-brain barrier in vitro",

abstract = "Interferon-β (IFN-β) stabilizes the blood-brain barrier (BBB) in vitro. Here we investigated the effect of serum from 15 IFN-β-1b-treated multiple sclerosis (MS) patients on the permeability read-outs of small solutes in an in vitro BBB model consisting of human brain microvascular endothelial cells in co-culture with rat astrocytes. The addition of sera from IFN-β-treated patients resulted in a significantly (p < 0.05) reduced permeability as compared with untreated patients. Our findings show that sera from IFN-β-1b-treated MS patients have a stabilizing effect on the in vitro BBB. We suggest an unknown potentially pro-inflammatory factor in the serum of MS patients that may lead to a BBB dysfunction and can be modulated by IFN-β.",

keywords = "bioassay, blood-brain barrier, interferon-β, multiple sclerosis",

author = "Marcus M{\"u}ller and Achim Frese and Isabelle Nassenstein and Maike Hoppen and Martin Marziniak and Ringelstein, {Erich Bernd} and Kim, {Kwang Sik} and Sch{\"a}bitz, {Wolf R{\"u}diger} and J{\"o}rg Kraus",

note = "Funding Information: This study was supported by Bayer Vital GmbH, Leverkusen, Germany, and by the IMF granting (projects KR120439 and KR2105 06) from the Medical Faculty, M{\"u}nster University, and by the Neuromedical Foundation (Stiftung Neuromedizin), M{\"u}nster.",

year = "2012",

month = feb,

doi = "10.1177/1352458511416837",

language = "English (US)",

volume = "18",

pages = "236--239",

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T1 - Serum from interferon-β-1b-treated patients with early multiple sclerosis stabilizes the blood-brain barrier in vitro

AU - Müller, Marcus

AU - Frese, Achim

AU - Nassenstein, Isabelle

AU - Hoppen, Maike

AU - Marziniak, Martin

AU - Ringelstein, Erich Bernd

AU - Kim, Kwang Sik

AU - Schäbitz, Wolf Rüdiger

AU - Kraus, Jörg

N1 - Funding Information: This study was supported by Bayer Vital GmbH, Leverkusen, Germany, and by the IMF granting (projects KR120439 and KR2105 06) from the Medical Faculty, Münster University, and by the Neuromedical Foundation (Stiftung Neuromedizin), Münster.

PY - 2012/2

Y1 - 2012/2

N2 - Interferon-β (IFN-β) stabilizes the blood-brain barrier (BBB) in vitro. Here we investigated the effect of serum from 15 IFN-β-1b-treated multiple sclerosis (MS) patients on the permeability read-outs of small solutes in an in vitro BBB model consisting of human brain microvascular endothelial cells in co-culture with rat astrocytes. The addition of sera from IFN-β-treated patients resulted in a significantly (p < 0.05) reduced permeability as compared with untreated patients. Our findings show that sera from IFN-β-1b-treated MS patients have a stabilizing effect on the in vitro BBB. We suggest an unknown potentially pro-inflammatory factor in the serum of MS patients that may lead to a BBB dysfunction and can be modulated by IFN-β.

AB - Interferon-β (IFN-β) stabilizes the blood-brain barrier (BBB) in vitro. Here we investigated the effect of serum from 15 IFN-β-1b-treated multiple sclerosis (MS) patients on the permeability read-outs of small solutes in an in vitro BBB model consisting of human brain microvascular endothelial cells in co-culture with rat astrocytes. The addition of sera from IFN-β-treated patients resulted in a significantly (p < 0.05) reduced permeability as compared with untreated patients. Our findings show that sera from IFN-β-1b-treated MS patients have a stabilizing effect on the in vitro BBB. We suggest an unknown potentially pro-inflammatory factor in the serum of MS patients that may lead to a BBB dysfunction and can be modulated by IFN-β.

KW - bioassay

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KW - interferon-β

KW - multiple sclerosis

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Serum from interferon-β-1b-treated patients with early multiple sclerosis stabilizes the blood-brain barrier in vitro

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