Sertoli cells, proximal convoluted tubules in the kidney, and neurons in the brain contain cyclic protein-2

S. D. Zabludoff, M. Erickson-Lawrence, W. W. Wright

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


We analyze by immunocytochemistry the in vivo distribution in rat Sertoli cells of Cyclic Protein-2 (CP-2), which is maximally synthesized and secreted in vitro at stages VI and VII of the cycle of the seminiferous epithelium. This analysis demonstrates that CP-2 staining is strongest in Sertoli cells in stage VI and VII tubules. Additionally, we demonstrate that the staining for CP-2 within a stage VII tubule differs from the staining of another Sertoli cell secretory product, androgen-binding protein. CP-2 is not detected by immunocytochemistry in any other tissues of the reproductive tract, though immunoblot analysis demonstrates the presence of CP-2 in rete testis and epididymal fluids. CP-2 was immunocytochemically detected in only three other organs: the kidney, the brain (with greatest concentration in the supraoptic and paraventricular nuclei), and the posterior pituitary. The presence of CP-2 in the kidney was confirmed by metabolic radiolabeling, immunoprecipitation, and peptide analysis. The presence of CP-2 in the brain was confirmed by immunoblot analysis of radioinert protein immunoprecipitated from the anterior hypothalamus.

Original languageEnglish (US)
Pages (from-to)15-22
Number of pages8
JournalBiology of reproduction
Issue number1
StatePublished - 1990
Externally publishedYes

ASJC Scopus subject areas

  • Reproductive Medicine
  • Cell Biology


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