Serotonin in human lumbar cerebrospinal fluid: A reassessment

George M. Anderson; Ivan N. Mefford; Teresa J. Tolliver; Mark A. Riddle; Dave M. Ocame; James F. Leckman; Donald J. Cohen

doi:10.1016/0024-3205(90)90030-U

Serotonin in human lumbar cerebrospinal fluid: A reassessment

George M. Anderson, Ivan N. Mefford, Teresa J. Tolliver, Mark A. Riddle, Dave M. Ocame, James F. Leckman, Donald J. Cohen

Research output: Contribution to journal › Article › peer-review

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Abstract

An inter-laboratory comparison study was carried out in order to ascertain mean levels of serotonin (5-HT) in human lumbar cerebrospinal fluid (CSF). Analyses were performed using high performance liquid chromatogrophy (HPLC) coupled with either electrochemical (LC-EC) or fluorometric (LC-F) detection. with the detection limits obtained (7-8 pg/ml for LC-EC, 7-15 pg/ml for LC-F) 5-HT was not usually detected in human lumbar CSF. The findings indicate that the true mean concentration of CSF 5-HT is less than 10 pg/ml. This upper limit is substantially lower than all previous reports of 5-HT concentrations in normal lumbar CSF. The extremely low concentrations of 5-HT present in CSF make it unlikely that CSF 5-HT will be of clinical utility in assessing central serotonergic function.

Original language	English (US)
Pages (from-to)	247-255
Number of pages	9
Journal	Life Sciences
Volume	46
Issue number	4
DOIs	https://doi.org/10.1016/0024-3205(90)90030-U
State	Published - 1990
Externally published	Yes

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Pharmacology, Toxicology and Pharmaceutics(all)

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10.1016/0024-3205(90)90030-U

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@article{bf0e5151ce3844c8a768fbca01ca66a9,

title = "Serotonin in human lumbar cerebrospinal fluid: A reassessment",

abstract = "An inter-laboratory comparison study was carried out in order to ascertain mean levels of serotonin (5-HT) in human lumbar cerebrospinal fluid (CSF). Analyses were performed using high performance liquid chromatogrophy (HPLC) coupled with either electrochemical (LC-EC) or fluorometric (LC-F) detection. with the detection limits obtained (7-8 pg/ml for LC-EC, 7-15 pg/ml for LC-F) 5-HT was not usually detected in human lumbar CSF. The findings indicate that the true mean concentration of CSF 5-HT is less than 10 pg/ml. This upper limit is substantially lower than all previous reports of 5-HT concentrations in normal lumbar CSF. The extremely low concentrations of 5-HT present in CSF make it unlikely that CSF 5-HT will be of clinical utility in assessing central serotonergic function.",

author = "Anderson, {George M.} and Mefford, {Ivan N.} and Tolliver, {Teresa J.} and Riddle, {Mark A.} and Ocame, {Dave M.} and Leckman, {James F.} and Cohen, {Donald J.}",

note = "Funding Information: The research was supported by MHCRC Grant MH30929, the Marks Foundation, the Gettner Research Fund, and the Lowenstein Foundation.",

year = "1990",

doi = "10.1016/0024-3205(90)90030-U",

language = "English (US)",

volume = "46",

pages = "247--255",

journal = "Life Sciences",

issn = "0024-3205",

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number = "4",

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TY - JOUR

T1 - Serotonin in human lumbar cerebrospinal fluid

T2 - A reassessment

AU - Anderson, George M.

AU - Mefford, Ivan N.

AU - Tolliver, Teresa J.

AU - Riddle, Mark A.

AU - Ocame, Dave M.

AU - Leckman, James F.

AU - Cohen, Donald J.

N1 - Funding Information: The research was supported by MHCRC Grant MH30929, the Marks Foundation, the Gettner Research Fund, and the Lowenstein Foundation.

PY - 1990

Y1 - 1990

N2 - An inter-laboratory comparison study was carried out in order to ascertain mean levels of serotonin (5-HT) in human lumbar cerebrospinal fluid (CSF). Analyses were performed using high performance liquid chromatogrophy (HPLC) coupled with either electrochemical (LC-EC) or fluorometric (LC-F) detection. with the detection limits obtained (7-8 pg/ml for LC-EC, 7-15 pg/ml for LC-F) 5-HT was not usually detected in human lumbar CSF. The findings indicate that the true mean concentration of CSF 5-HT is less than 10 pg/ml. This upper limit is substantially lower than all previous reports of 5-HT concentrations in normal lumbar CSF. The extremely low concentrations of 5-HT present in CSF make it unlikely that CSF 5-HT will be of clinical utility in assessing central serotonergic function.

AB - An inter-laboratory comparison study was carried out in order to ascertain mean levels of serotonin (5-HT) in human lumbar cerebrospinal fluid (CSF). Analyses were performed using high performance liquid chromatogrophy (HPLC) coupled with either electrochemical (LC-EC) or fluorometric (LC-F) detection. with the detection limits obtained (7-8 pg/ml for LC-EC, 7-15 pg/ml for LC-F) 5-HT was not usually detected in human lumbar CSF. The findings indicate that the true mean concentration of CSF 5-HT is less than 10 pg/ml. This upper limit is substantially lower than all previous reports of 5-HT concentrations in normal lumbar CSF. The extremely low concentrations of 5-HT present in CSF make it unlikely that CSF 5-HT will be of clinical utility in assessing central serotonergic function.

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DO - 10.1016/0024-3205(90)90030-U

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SN - 0024-3205

VL - 46

SP - 247

EP - 255

JO - Life Sciences

JF - Life Sciences

IS - 4

ER -

Serotonin in human lumbar cerebrospinal fluid: A reassessment

Abstract

ASJC Scopus subject areas

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