Serotonin axons in the neocortex of the adult female mouse regrow after traumatic brain injury

Tymoteusz J. Kajstura, Sarah E. Dougherty, David J. Linden

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


It is widely held that injured neurons in the central nervous system do not undergo axonal regrowth. However, there is mounting evidence that serotonin axons are a notable exception. Serotonin axons undergo long-distance regrowth in the neocortex after amphetamine lesion, and, following a penetrating stab injury, they can regrow from cut ends to traverse the stab rift. Traumatic brain injury (TBI) is clinically prevalent and can lead to pathologies, such as depression, that are related to serotonergic dysfunction. Thus, whether serotonin axons can regrow after TBI is an important question. We used two models for TBI—a persistent open skull condition and controlled cortical impact—to evoke injury in adult female mouse neocortex, and assessed serotonin axon density 1 week, 1 month, and 3 months after injury by serotonin transporter immunohistochemistry. We found that after both forms of TBI, serotonin axon density is decreased posterior but not anterior to the injury site when measured in layer 1 at 1 week post surgery, and that serotonin axons are capable of regrowing into the distal zone to increase density by 1 month post surgery. This pattern is consistent with the anterior-to-posterior course of serotonin axons in the neocortex. TBI in these models is associated with significant reactive astrogliosis both anterior and posterior to the impact, but the degree of reactive astrogliosis is not correlated with serotonin axon density when measured 1 week after TBI. Microglial density remains constant following both types of injuries, but microglial condensation was detected 1 week after controlled cortical impact.

Original languageEnglish (US)
Pages (from-to)512-526
Number of pages15
JournalJournal of neuroscience research
Issue number4
StatePublished - Apr 2018


  • controlled cortical impact
  • regeneration
  • serotonin
  • traumatic brain injury

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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