Seroprevalence of Antibodies against Highly Pathogenic Avian Influenza A (H5N1) Virus among Poultry Workers in Bangladesh, 2009

Sharifa Nasreen; Salah Uddin Khan; Eduardo Azziz-Baumgartner; Kathy Hancock; Vic Veguilla; David Wang; Mahmudur Rahman; A. S.M. Alamgir; Katharine Sturm-Ramirez; Emily S. Gurley; Stephen P. Luby; Jacqueline M. Katz; Timothy M. Uyeki

doi:10.1371/journal.pone.0073200

Seroprevalence of Antibodies against Highly Pathogenic Avian Influenza A (H5N1) Virus among Poultry Workers in Bangladesh, 2009

Sharifa Nasreen, Salah Uddin Khan, Eduardo Azziz-Baumgartner, Kathy Hancock, Vic Veguilla, David Wang, Mahmudur Rahman, A. S.M. Alamgir, Katharine Sturm-Ramirez, Emily S. Gurley, Stephen P. Luby, Jacqueline M. Katz, Timothy M. Uyeki

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Abstract

We conducted a cross-sectional study in 2009 to determine the seroprevalence and risk factors for highly pathogenic avian influenza A (H5N1) [HPAI H5N1] virus antibodies among poultry workers at farms and live bird markets with confirmed/suspected poultry outbreaks during 2009 in Bangladesh. We tested sera by microneutralization assay using A/Bangladesh/207095/2008 (H5N1; clade 2.2.2) virus with confirmation by horse red blood cell hemagglutination inhibition and H5-specific Western blot assays. We enrolled 212 workers from 87 farms and 210 workers from three live bird markets. One hundred and two farm workers (48%) culled poultry. One hundred and ninety-three farm workers (91%) and 178 market workers (85%) reported direct contact with poultry that died during a laboratory confirmed HPAI H5N1 poultry farm outbreak or market poultry die-offs from suspected HPAI H5N1. Despite exposure to sick poultry, no farm or market poultry workers were seropositive for HPAI H5N1 virus antibodies (95% confidence interval 0-1%).

Original language	English (US)
Article number	e73200
Journal	PloS one
Volume	8
Issue number	9
DOIs	https://doi.org/10.1371/journal.pone.0073200
State	Published - Sep 5 2013
Externally published	Yes

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Nasreen, S., Uddin Khan, S., Azziz-Baumgartner, E., Hancock, K., Veguilla, V., Wang, D., Rahman, M., Alamgir, A. S. M., Sturm-Ramirez, K., Gurley, E. S., Luby, S. P., Katz, J. M., & Uyeki, T. M. (2013). Seroprevalence of Antibodies against Highly Pathogenic Avian Influenza A (H5N1) Virus among Poultry Workers in Bangladesh, 2009. PloS one, 8(9), Article e73200. https://doi.org/10.1371/journal.pone.0073200

Nasreen, S, Uddin Khan, S, Azziz-Baumgartner, E, Hancock, K, Veguilla, V, Wang, D, Rahman, M, Alamgir, ASM, Sturm-Ramirez, K, Gurley, ES, Luby, SP, Katz, JM & Uyeki, TM 2013, 'Seroprevalence of Antibodies against Highly Pathogenic Avian Influenza A (H5N1) Virus among Poultry Workers in Bangladesh, 2009', PloS one, vol. 8, no. 9, e73200. https://doi.org/10.1371/journal.pone.0073200

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abstract = "We conducted a cross-sectional study in 2009 to determine the seroprevalence and risk factors for highly pathogenic avian influenza A (H5N1) [HPAI H5N1] virus antibodies among poultry workers at farms and live bird markets with confirmed/suspected poultry outbreaks during 2009 in Bangladesh. We tested sera by microneutralization assay using A/Bangladesh/207095/2008 (H5N1; clade 2.2.2) virus with confirmation by horse red blood cell hemagglutination inhibition and H5-specific Western blot assays. We enrolled 212 workers from 87 farms and 210 workers from three live bird markets. One hundred and two farm workers (48%) culled poultry. One hundred and ninety-three farm workers (91%) and 178 market workers (85%) reported direct contact with poultry that died during a laboratory confirmed HPAI H5N1 poultry farm outbreak or market poultry die-offs from suspected HPAI H5N1. Despite exposure to sick poultry, no farm or market poultry workers were seropositive for HPAI H5N1 virus antibodies (95% confidence interval 0-1%).",

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Seroprevalence of Antibodies against Highly Pathogenic Avian Influenza A (H5N1) Virus among Poultry Workers in Bangladesh, 2009

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