Serological and molecular survey of Anaplasma species infection in dogs with suspected tickborne disease in Portugal

A. S. Santos, N. Alexandre, R. Sousa, M. S. Núncio, F. Bacellar, J. S. Dumler

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Fifty-five dogs with suspected tickborne disease were tested by immunofluorescence assay and PCR for Anaplasma phagocytophilum infection. Thirty (54-5 per cent) of the dogs were seropositive and five of them fulfilled the serological criteria for an active infection, with either seroconversion or a fourfold increase in antibody titres. Fragments of DNA of the expected size were detected by PCR in two seropositive and three seronegative dogs. However, direct amplicon sequencing failed to identify active A phagocytophilum infections, but revealed the presence of Anaplasma platys DNA in the PCR-positive animals.

Original languageEnglish (US)
Pages (from-to)168-171
Number of pages4
JournalVeterinary Record
Volume164
Issue number6
DOIs
StatePublished - Feb 7 2009
Externally publishedYes

ASJC Scopus subject areas

  • General Veterinary

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