TY - JOUR
T1 - Sernaphorin III can function as a selective chemorepellent to pattern sensory projections in the spinal cord
AU - Messersmith, Elizabeth K.
AU - Leonardo, E. David
AU - Shatz, Carla J.
AU - Tessier-Lavigne, Marc
AU - Goodman, Corey S.
AU - Kolodkin, Alex L.
N1 - Funding Information:
Correspondence should be addressed to M. T.-L. We thank Sophia Colamarino for critical comments on the manuscript, Virginia Lee for the generous gift of NF-M, David Shelton for the generous gift of NT-3, and Jay Rehm, Krystyna Kilomanski, Caroline Esmurdoc, and Larry Nelson for technical assistance. We thank William Snider, Doug Wright, and their colleagues for communication of results prior to publication. E. D. L. was supported by NIH grant GM07618 through the UCSF MSTP program. E. K. M. was supported by the March of Dimes Birth Defects Foundation (to C. J. S.) and by Fight for Sight National Society to Prevent Blindness Postdoctoral Fellowship (PD93007). M. T.-L. was supported by the Lucille P. Markey Charitable Trust. A. L. K. was supported by NIH grant HD21294 (to C. S. G.). C. J. S. and C. S. G. are Investigators and M. T.-L. is an Assistant Investigator with the Howard Hughes Medical Institute.
PY - 1995/5
Y1 - 1995/5
N2 - Distinct classes of primary sensory neurons in dorsal root ganglia subserve different sensory modalities, terminate in different dorsoventral locations in the spinal cord, and display different neurotrophin response profiles. Large diameter muscle afferents that terminate in the ventral spinal cord are NT-3 responsive, whereas small diameter afferents subserving pain and temperature are NGF responsive and terminate in the dorsal spinal cord. Previous in vitro studies showed that the developing ventral spinal cord secretes a diffusible factor that inhibits the growth of sensory axons. Here we show that this factor repels NGF-responsive axons but has little effect on NT-3-responsive axons. We also provide evidence implicating semaphorin III/collapsin, a diffusible guidance molecule expressed by ventral spinal cord cells, in mediating this effect. These results suggest that semaphorin III functions to pattern sensory projections by selectively repelling axons that normally terminate dorsally.
AB - Distinct classes of primary sensory neurons in dorsal root ganglia subserve different sensory modalities, terminate in different dorsoventral locations in the spinal cord, and display different neurotrophin response profiles. Large diameter muscle afferents that terminate in the ventral spinal cord are NT-3 responsive, whereas small diameter afferents subserving pain and temperature are NGF responsive and terminate in the dorsal spinal cord. Previous in vitro studies showed that the developing ventral spinal cord secretes a diffusible factor that inhibits the growth of sensory axons. Here we show that this factor repels NGF-responsive axons but has little effect on NT-3-responsive axons. We also provide evidence implicating semaphorin III/collapsin, a diffusible guidance molecule expressed by ventral spinal cord cells, in mediating this effect. These results suggest that semaphorin III functions to pattern sensory projections by selectively repelling axons that normally terminate dorsally.
UR - http://www.scopus.com/inward/record.url?scp=0029037182&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029037182&partnerID=8YFLogxK
U2 - 10.1016/0896-6273(95)90333-X
DO - 10.1016/0896-6273(95)90333-X
M3 - Article
C2 - 7748562
AN - SCOPUS:0029037182
SN - 0896-6273
VL - 14
SP - 949
EP - 959
JO - Neuron
JF - Neuron
IS - 5
ER -