Abstract
Plasma antithrombin-III (AT-III), protein S, and protein C were measured during myocardial stunning (MS) and acute myocardial infarction (AMI). The effects of magnesium (Mg), diltiazem, and a Mac-1 inhibitor on their plasma levels were elucidated. Forty-nine open-chest swine underwent brief (8 min), or prolonged (50 min) coronary artery occlusion followed by reperfusion. During MS an increase in the plasma AT-III (from 98.5 ± 3.38% to 138.1 ± 3.6%) during the early occlusion phase, without any further changes was observed. The profile of total protein S was not changed during MS. Protein C increased at the end of occlusion (from 45.3 ± 1.8% to 55.7 ± 1.4%) reaching a peak (64.5 ± 1.4%) at the beginning of reperfusion. When compared with controls, no significant differences were found in the antithrombotics profile during MS after pretreatment with Mac-1 inhibitor. For the AMI, the AT-III decreased during occlusion (from 98.5 ± 3.4% to 61.0 ± 3.6%). The protein S decreased during occlusion with the lowest level at 1 h of reperfusion (from 71.8 ± 2.2% to 46.7 ± 1.0%), followed by an increase during late reperfusion (59.2 ± 1.5%). Contrarily, protein C increased during occlusion and early reperfusion(from 44.7 ± 2.6% to 79.4 ± 2.4%), but declined to 49.6 ± 2.5% thereafter. In both Mg and diltiazem-treated swine, protein C was higher at the end of occlusion and during the entire reperfusion period compared with controls. Mg and diltiazem therapy was associated with the slight elevation of plasma AT-III. The patterns for protein S level during ischemia-reperfusion were similar with the controls. Protein S was higher at the end of occlusion and through the entire reperfusion in the NPC 15669-treated animals when compared with the controls. Mac-1 inhibition was associated with the elevated protein C during late reperfusion. Ability of Mg, diltiazem, and a Mac-1 inhibitor to favorably modulate the plasma level of antithrombotics have direct clinical implications for the use of these agents in patients with acute coronary artery syndromes.
Original language | English (US) |
---|---|
Pages (from-to) | 632-640 |
Number of pages | 9 |
Journal | Blood Coagulation and Fibrinolysis |
Volume | 7 |
Issue number | 6 |
DOIs | |
State | Published - Jan 1 1996 |
Keywords
- Mac-1 inhibitor
- acute myocardial infarction
- animal model
- antithrombin III
- diltiazem
- magnesium
- myocardial stunning
- protein C
- protein S
ASJC Scopus subject areas
- Hematology