TY - JOUR
T1 - Serial analysis of gene expression in the chicken otocyst
AU - Sinkkonen, Saku T.
AU - Starlinger, Veronika
AU - Galaiya, Deepa J.
AU - Laske, Roman D.
AU - Myllykangas, Samuel
AU - Oshima, Kazuo
AU - Heller, Stefan
N1 - Funding Information:
This project was supported by the Sigrid Jusélius Foundation and Instrumentarium Science Foundation (to S.T.S.), a Stanford Dean’s Fellowship, and by fellowship D/06/41764 from the German Academic Exchange Service (to V.S.), as well as grants DC006167, DC010042, and P30 DC010363 from the National Institutes of Health (to S.H.).
PY - 2011/12
Y1 - 2011/12
N2 - The inner ear arises from multipotent placodal precursors that are gradually committed to the otic fate and further differentiate into all inner ear cell types, with the exception of a few immigrating neural crest-derived cells. The otocyst plays a pivotal role during inner ear development: Otic progenitor cells sub-compartmentalize into non-sensory and prosensory domains, giving rise to individual vestibular and auditory organs and their associated ganglia. The genes and pathways underlying this progressive subdivision and differentiation process are not entirely known. The goal of this study was to identify a comprehensive set of genes expressed in the chicken otocyst using the serial analysis of gene expression (SAGE) method. Our analysis revealed several hundred transcriptional regulators, potential signaling proteins, and receptors. We identified a substantial collection of genes that were previously known in the context of inner ear development, but we also found many new candidate genes, such as SOX4, SOX5, SOX7, SOX8, SOX11, and SOX18, which previously were not known to be expressed in the developing inner ear. Despite its limitation of not being all-inclusive, the generated otocyst SAGE library is a practical bioinformatics tool to study otocyst gene expression and to identify candidate genes for developmental studies.
AB - The inner ear arises from multipotent placodal precursors that are gradually committed to the otic fate and further differentiate into all inner ear cell types, with the exception of a few immigrating neural crest-derived cells. The otocyst plays a pivotal role during inner ear development: Otic progenitor cells sub-compartmentalize into non-sensory and prosensory domains, giving rise to individual vestibular and auditory organs and their associated ganglia. The genes and pathways underlying this progressive subdivision and differentiation process are not entirely known. The goal of this study was to identify a comprehensive set of genes expressed in the chicken otocyst using the serial analysis of gene expression (SAGE) method. Our analysis revealed several hundred transcriptional regulators, potential signaling proteins, and receptors. We identified a substantial collection of genes that were previously known in the context of inner ear development, but we also found many new candidate genes, such as SOX4, SOX5, SOX7, SOX8, SOX11, and SOX18, which previously were not known to be expressed in the developing inner ear. Despite its limitation of not being all-inclusive, the generated otocyst SAGE library is a practical bioinformatics tool to study otocyst gene expression and to identify candidate genes for developmental studies.
KW - Gene array
KW - Inner ear development
KW - Otic vesicle
KW - SAGE
KW - Sox
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U2 - 10.1007/s10162-011-0286-z
DO - 10.1007/s10162-011-0286-z
M3 - Article
C2 - 21853378
AN - SCOPUS:84855711608
SN - 1525-3961
VL - 12
SP - 697
EP - 710
JO - JARO - Journal of the Association for Research in Otolaryngology
JF - JARO - Journal of the Association for Research in Otolaryngology
IS - 6
ER -