Abstract
Objective To explore a method to separate and purify conveniently and efficiently apolipoprotein H from human plasma. Methods We added perchloric acid into human plasma. Apolipoprotein H was separated and purified by ion exchange chromatography and affinity chromatography. The purified apolipoprotein H was analyzed by SDS-polyacrylamide gel electrophoresis, Western blot and BCA kit. Results We repeated the experiment three times. The purity of the apolipoprotein H was all over 98%. Western blot demonstrated that anti β2- Glycoprotein I monoclonal antibody reacted with the purified apolipoprotein H. We obtained a total of 12. 56 mg purified apolipoprotein H, average 21 mg/L. Conclusions This method has good repeatability, reliability and more convenient and economical than the traditional method.
Original language | English (US) |
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Pages (from-to) | 61-64 |
Number of pages | 4 |
Journal | Fudan University Journal of Medical Sciences |
Volume | 36 |
Issue number | 1 |
State | Published - Jan 2009 |
Externally published | Yes |
Keywords
- Apolipoprotein H
- Identify
- Purify
- Separate
ASJC Scopus subject areas
- Medicine(all)