TY - JOUR
T1 - Separation of major prostaglandins, leukotrienes, and monoHETEs by high performance liquid chromatography
AU - Peters, Stephen P.
AU - Schulman, Edward S.
AU - Liu, Mark C.
AU - Hayes, Edward C.
AU - Lichtenstein, Lawrence M.
N1 - Funding Information:
i Supported by Grants AI 07290 and HL 23586 from the National Institute of Allergy and Infectious Diseases, and the National Heart, Lung and Blood Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, U.S.A. 2 Publication no. 535 from the O'Neill Laboratories, The Good Samaritan Hospital, Baltimore, MD, U.S.A. 3 Recipient of Clinical Investigator Award 1 K08 HL 01034 from the National Heart, Lung, and Blood Institute, and to whom correspondence should be addressed at The Johns Hopkins University School of Medicine, Division of Clinical Immunology at The Good Samaritan Hospital, 5601 Loch Raven Blvd., Baltimore, MD 21239, U.S.A. 4 Supported in part by National Pulmonary Faculty Training Center Grant HL 07159 from the National Heart, Lung and Blood Institute. Present address: Division of Pulmonary Diseases, Department of Medicine, Thomas Jefferson University Medical Center, Philadelphia, PA, U.S.A.
PY - 1983/11/25
Y1 - 1983/11/25
N2 - A procedure using high performance liquid chromatography (HPLC) is described for the separation of major primary cyclooxygenase metabolites (prostacyclin metabolite-6ketoPGF1α, thromboxane B2, and prostaglandins F2α, E2, and D2), leukotrienes (C4, B4, and D4), monohydroxyeicosatetraenoic acids (15-, 11-, 12-, and 5HETEs), and free arachidonic acid. It is therefore possible to quantitate major arachidonic acid metabolites by a single chromatographic procedure. Using this technique we have determined that a major arachidonic acid metabolite of human lung macrophages co-elutes with leukotriene B4.
AB - A procedure using high performance liquid chromatography (HPLC) is described for the separation of major primary cyclooxygenase metabolites (prostacyclin metabolite-6ketoPGF1α, thromboxane B2, and prostaglandins F2α, E2, and D2), leukotrienes (C4, B4, and D4), monohydroxyeicosatetraenoic acids (15-, 11-, 12-, and 5HETEs), and free arachidonic acid. It is therefore possible to quantitate major arachidonic acid metabolites by a single chromatographic procedure. Using this technique we have determined that a major arachidonic acid metabolite of human lung macrophages co-elutes with leukotriene B4.
KW - HETEs
KW - HPLC
KW - arachidonic acid
KW - human lung macrophages
KW - leukotrienes
KW - prostaglandins
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U2 - 10.1016/0022-1759(83)90441-6
DO - 10.1016/0022-1759(83)90441-6
M3 - Article
C2 - 6321597
AN - SCOPUS:0021076155
SN - 0022-1759
VL - 64
SP - 335
EP - 343
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 3
ER -