Separation and identification of compounds in Adinandra nitida by comprehensive two-dimensional liquid chromatography coupled to atmospheric pressure chemical ionization source ion trap tandem mass spectrometry

Jie Zhang, Dingyin Tao, Jicheng Duan, Zhen Liang, Weibing Zhang, Lihua Zhang, Yushu Huo, Yukui Zhang

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

A comprehensive two-dimensional liquid chromatographic (2D-LC) separation system based on the combination of a CN column and a Merck Chromolith Flash reversed-phase column was developed for the separation of components in Adinandra nitida, one type of traditional Chinese medicine (TCM). The two dimensions were connected by a ten-port, dual-position valve controlled automatically by software written in-house. The effluents were detected by both ultraviolet and atmospheric pressure chemical ionization source ion trap tandem mass spectrometry (MS). The calculated peak capacity of the 2D-LC-MS/MS system was above 1240. More than 57 components were resolved in the methanol extract from Adinandra nitida leaves, and five of these were identified based on their relative retention times, molecular weights and MS/MS spectra.

Original languageEnglish (US)
Pages (from-to)586-593
Number of pages8
JournalAnalytical and Bioanalytical Chemistry
Volume386
Issue number3
DOIs
StatePublished - Oct 2006
Externally publishedYes

Keywords

  • Adinandra nitida
  • Comprehensive two-dimensional liquid chromatography
  • Mass spectrometer
  • Monolithic column
  • Traditional Chinese medicine

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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