TY - JOUR
T1 - Sensitivity and specificity of HIV-1 testing of urine compared with serum specimens
T2 - Rakai, Uganda
AU - Meehan, Mary P.
AU - Sewankambo, Nelson K.
AU - Wawer, Maria J.
AU - McNairn, Denise
AU - Quinn, Thomas C.
AU - Lutalo, Thomas
AU - Kalibbala, Sarah
AU - Li, Chuanjun
AU - Serwadda, David
AU - Wabwire-Mangen, Fred
AU - Kiwanuka, Noah
AU - Kigozi, Godfrey
AU - Kidugavu, Medi
AU - Gray, Ronald H.
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1999/11
Y1 - 1999/11
N2 - Background and Objectives: To evaluate a urine HIV-1 test. Study Design: Paired urine and blood samples from a sample of 222 subjects were assayed for HIV-1 using Calypte HIV-1 Urine enzyme immunoassay (EIA) with Western blot (WB) confirmation, and sera were tested by EIA and WB. Masked assays were done on stored, refrigerated urine at Johns Hopkins University (JH), and on fresh specimens at the Rakai Project, Entebbe (RP). We assessed the sensitivity and specificity of the urine relative to serum assays. Results: Compliance with provision of urine samples (95.0%) was higher than provision of serum (90.5%). Ninety-six sera were HIV-positive; 92 were HIV-positive on stored urine at JH (sensitivity 95.8%, CI 91.8-99.8%); and 94 (100%) were positive on fresh samples at the RP laboratory (sensitivity = 100.0%). Among serum HIV-negative subjects, all frozen urine were negative at JH and 97.7% of fresh samples were negative at RP. Conclusions: The Calypte urine HIV-1 EIA with WB is sensitive and specific. In this population, provision of urine was more acceptable than provision of blood samples.
AB - Background and Objectives: To evaluate a urine HIV-1 test. Study Design: Paired urine and blood samples from a sample of 222 subjects were assayed for HIV-1 using Calypte HIV-1 Urine enzyme immunoassay (EIA) with Western blot (WB) confirmation, and sera were tested by EIA and WB. Masked assays were done on stored, refrigerated urine at Johns Hopkins University (JH), and on fresh specimens at the Rakai Project, Entebbe (RP). We assessed the sensitivity and specificity of the urine relative to serum assays. Results: Compliance with provision of urine samples (95.0%) was higher than provision of serum (90.5%). Ninety-six sera were HIV-positive; 92 were HIV-positive on stored urine at JH (sensitivity 95.8%, CI 91.8-99.8%); and 94 (100%) were positive on fresh samples at the RP laboratory (sensitivity = 100.0%). Among serum HIV-negative subjects, all frozen urine were negative at JH and 97.7% of fresh samples were negative at RP. Conclusions: The Calypte urine HIV-1 EIA with WB is sensitive and specific. In this population, provision of urine was more acceptable than provision of blood samples.
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U2 - 10.1097/00007435-199911000-00009
DO - 10.1097/00007435-199911000-00009
M3 - Article
C2 - 10560724
AN - SCOPUS:0032694030
SN - 0148-5717
VL - 26
SP - 590
EP - 592
JO - Sexually transmitted diseases
JF - Sexually transmitted diseases
IS - 10
ER -