Semaphorin 6B acts as a receptor in post-crossing commissural axon guidance

Irwin Andermatt, Nicole H. Wilson, Timothy Bergmann, Olivier Mauti, Matthias Gesemann, Shanthini Sockanathan, Esther T. Stoeckli

Research output: Contribution to journalArticlepeer-review

43 Scopus citations


Semaphorins are a large family of axon guidance molecules that are known primarily as ligands for plexins and neuropilins. Although class-6 semaphorins are transmembrane proteins, they have been implicated as ligands in different aspects of neural development, including neural crest cell migration, axon guidance and cerebellar development. However, the specific spatial and temporal expression of semaphorin 6B (Sema6B) in chick commissural neurons suggested a receptor role in axon guidance at the spinal cord midline. Indeed, in the absence of Sema6B, post-crossing commissural axons lacked an instructive signal directing them rostrally along the contralateral floorplate border, resulting in stalling at the exit site or even caudal turns. Truncated Sema6B lacking the intracellular domain was unable to rescue the loss-of-function phenotype, confirming a receptor function of Sema6B. In support of this, we demonstrate that Sema6B binds to floorplate-derived plexin A2 (PlxnA2) for navigation at the midline, whereas a cis-interaction between PlxnA2 and Sema6B on pre-crossing commissural axons may regulate the responsiveness of axons to floorplate-derived cues.

Original languageEnglish (US)
Pages (from-to)3709-3720
Number of pages12
JournalDevelopment (Cambridge)
Issue number19
StatePublished - Oct 1 2014


  • Chick
  • Cis-interaction
  • In ovo RNAi
  • PlexinA2
  • Spinal cord development

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology


Dive into the research topics of 'Semaphorin 6B acts as a receptor in post-crossing commissural axon guidance'. Together they form a unique fingerprint.

Cite this