Self-assembled monolayers improve protein distribution on holey carbon cryo-EM supports

Joel R. Meyerson; Prashant Rao; Janesh Kumar; Sagar Chittori; Soojay Banerjee; Jason Pierson; Mark L. Mayer; Sriram Subramaniam

doi:10.1038/srep07084

Self-assembled monolayers improve protein distribution on holey carbon cryo-EM supports

Joel R. Meyerson, Prashant Rao, Janesh Kumar, Sagar Chittori, Soojay Banerjee, Jason Pierson, Mark L. Mayer, Sriram Subramaniam

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

Poor partitioning of macromolecules into the holes of holey carbon support grids frequently limits structural determination by single particle cryo-electron microscopy (cryo-EM). Here, we present a method to deposit, on gold-coated carbon grids, a self-assembled monolayer whose surface properties can be controlled by chemical modification. We demonstrate the utility of this approach to drive partitioning of ionotropic glutamate receptors into the holes, thereby enabling 3D structural analysis using cryo-EM methods.

Original language	English (US)
Article number	7084
Journal	Scientific Reports
Volume	4
DOIs	https://doi.org/10.1038/srep07084
State	Published - Nov 18 2014
Externally published	Yes

ASJC Scopus subject areas

General
Medicine(all)

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abstract = "Poor partitioning of macromolecules into the holes of holey carbon support grids frequently limits structural determination by single particle cryo-electron microscopy (cryo-EM). Here, we present a method to deposit, on gold-coated carbon grids, a self-assembled monolayer whose surface properties can be controlled by chemical modification. We demonstrate the utility of this approach to drive partitioning of ionotropic glutamate receptors into the holes, thereby enabling 3D structural analysis using cryo-EM methods.",

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AU - Meyerson, Joel R.

AU - Rao, Prashant

AU - Kumar, Janesh

AU - Chittori, Sagar

AU - Banerjee, Soojay

AU - Pierson, Jason

AU - Mayer, Mark L.

AU - Subramaniam, Sriram

PY - 2014/11/18

Y1 - 2014/11/18

N2 - Poor partitioning of macromolecules into the holes of holey carbon support grids frequently limits structural determination by single particle cryo-electron microscopy (cryo-EM). Here, we present a method to deposit, on gold-coated carbon grids, a self-assembled monolayer whose surface properties can be controlled by chemical modification. We demonstrate the utility of this approach to drive partitioning of ionotropic glutamate receptors into the holes, thereby enabling 3D structural analysis using cryo-EM methods.

AB - Poor partitioning of macromolecules into the holes of holey carbon support grids frequently limits structural determination by single particle cryo-electron microscopy (cryo-EM). Here, we present a method to deposit, on gold-coated carbon grids, a self-assembled monolayer whose surface properties can be controlled by chemical modification. We demonstrate the utility of this approach to drive partitioning of ionotropic glutamate receptors into the holes, thereby enabling 3D structural analysis using cryo-EM methods.

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Self-assembled monolayers improve protein distribution on holey carbon cryo-EM supports

Abstract

ASJC Scopus subject areas

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