Selective neurohormonal interactions in islet cell secretion in the isolated perfused human pancreas

F. Charles Brunicardi, Paul Druck, Neal E. Seymour, You Su Sun, Dariush Elahi, Dana K. Andersen

Research output: Contribution to journalArticlepeer-review

28 Scopus citations


To investigate the effects of stimulant interactions on α- and β-cell secretions, the differential effects of gastric inhibitory polypeptide (GIP) and cholinergic stimulation (CS) on insulin (IRI) and glucagon (IRG) release were examined during euglycemic, single-pass perfusion in the isolated human pancreas. Pancreata obtained from 12 cadaver organ donors were perfused for 15-min test periods with (a) 1 nM GIP (b) intrinsic CS via bipolar electrical stimulation (10 V, 5 msec, 10 Hz) of the splanchnic neural fibers during simultaneous perfusion with 4 μM phentolamine and 6 μM propranolol, or (c) GIP and CS. The integrated response of IRI and IRG demonstrated that IRI release was stimulated 308 ± 52 μU/g-min by GIP, 366 ± 73 μU/g-min by CS, and 560 ± 50 μU/g-min by GIP and CS (P < 0.05). IRG release was stimulated 111 ± 33 pg/g-min by GIP, 34 ± 12 pg/g-min by CS, and 90 ± 36 pg/g-min by GIP and CS. Combined hormonal and cholinergic stimulation was additive for IRI release, but not for IRG release. We conclude that the interaction of neural and hormonal islet cell stimuli is cell-type specific. This may result in selective impairment of hormone release after pancreatic denervation.

Original languageEnglish (US)
Pages (from-to)273-278
Number of pages6
JournalJournal of Surgical Research
Issue number4
StatePublished - Apr 1990
Externally publishedYes

ASJC Scopus subject areas

  • Surgery


Dive into the research topics of 'Selective neurohormonal interactions in islet cell secretion in the isolated perfused human pancreas'. Together they form a unique fingerprint.

Cite this