Selective detection of 2-deoxribonolactone in DNA

Kousuke Sato; Marc M. Greenberg

doi:10.1021/ja0426185

Selective detection of 2-deoxribonolactone in DNA

Kousuke Sato, Marc M. Greenberg

Krieger School of Arts and Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

2-Deoxyribonolactone (L) is an oxidized abasic lesion that is produced by a variety of DNA damaging agents. It exhibits unique biological effects with respect to its proclivity to form DNA-protein cross-links and promutagenic base pairs. Recent evidence suggests that the levels of this lesion caused by oxidative stress are underestimated. We have developed a simple, selective method for detecting subpicomole amounts of L in DNA. The method takes advantage of the selective reaction of the butenolide (2) derived from β-elimination from L with a biotinylated derivative of cysteine. This method will be useful for analyzing the levels of this oxidized abasic site in DNA.

Original language	English (US)
Pages (from-to)	2806-2807
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	127
Issue number	9
DOIs	https://doi.org/10.1021/ja0426185
State	Published - Mar 9 2005

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

Access to Document

10.1021/ja0426185

Cite this

@article{8315b6b8b0294f4b93c42fce521e834f,

title = "Selective detection of 2-deoxribonolactone in DNA",

abstract = "2-Deoxyribonolactone (L) is an oxidized abasic lesion that is produced by a variety of DNA damaging agents. It exhibits unique biological effects with respect to its proclivity to form DNA-protein cross-links and promutagenic base pairs. Recent evidence suggests that the levels of this lesion caused by oxidative stress are underestimated. We have developed a simple, selective method for detecting subpicomole amounts of L in DNA. The method takes advantage of the selective reaction of the butenolide (2) derived from β-elimination from L with a biotinylated derivative of cysteine. This method will be useful for analyzing the levels of this oxidized abasic site in DNA.",

author = "Kousuke Sato and Greenberg, {Marc M.}",

year = "2005",

month = mar,

day = "9",

doi = "10.1021/ja0426185",

language = "English (US)",

volume = "127",

pages = "2806--2807",

journal = "Journal of the American Chemical Society",

issn = "0002-7863",

publisher = "American Chemical Society",

number = "9",

}

TY - JOUR

T1 - Selective detection of 2-deoxribonolactone in DNA

AU - Sato, Kousuke

AU - Greenberg, Marc M.

PY - 2005/3/9

Y1 - 2005/3/9

N2 - 2-Deoxyribonolactone (L) is an oxidized abasic lesion that is produced by a variety of DNA damaging agents. It exhibits unique biological effects with respect to its proclivity to form DNA-protein cross-links and promutagenic base pairs. Recent evidence suggests that the levels of this lesion caused by oxidative stress are underestimated. We have developed a simple, selective method for detecting subpicomole amounts of L in DNA. The method takes advantage of the selective reaction of the butenolide (2) derived from β-elimination from L with a biotinylated derivative of cysteine. This method will be useful for analyzing the levels of this oxidized abasic site in DNA.

AB - 2-Deoxyribonolactone (L) is an oxidized abasic lesion that is produced by a variety of DNA damaging agents. It exhibits unique biological effects with respect to its proclivity to form DNA-protein cross-links and promutagenic base pairs. Recent evidence suggests that the levels of this lesion caused by oxidative stress are underestimated. We have developed a simple, selective method for detecting subpicomole amounts of L in DNA. The method takes advantage of the selective reaction of the butenolide (2) derived from β-elimination from L with a biotinylated derivative of cysteine. This method will be useful for analyzing the levels of this oxidized abasic site in DNA.

UR - http://www.scopus.com/inward/record.url?scp=14844296390&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=14844296390&partnerID=8YFLogxK

U2 - 10.1021/ja0426185

DO - 10.1021/ja0426185

M3 - Article

C2 - 15740088

AN - SCOPUS:14844296390

SN - 0002-7863

VL - 127

SP - 2806

EP - 2807

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 9

ER -

Selective detection of 2-deoxribonolactone in DNA

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this