TY - JOUR
T1 - Screening for coeliac disease in preschool Greek children
T2 - The feasibility study of a community-based project
AU - Karagiozoglou-Lampoudi, Thomai
AU - Zellos, Aglaia
AU - Vlahavas, George
AU - Kafritsa, Yota
AU - Roma, Eleftheria
AU - Papadopoulou, Alexandra
AU - Fotoulaki, Maria
AU - Karyda, Stavroula
AU - Xinias, Ioannis
AU - Savvidou, Afroditi
PY - 2013
Y1 - 2013
N2 - Aim Evaluation of the prevalence of coeliac disease (CD) in Greek paediatric population. Methods The project consists of two parts: (i) a pilot study of preschool children aged 2-6 years to test the feasibility and diagnostic accuracy of community-based screening and (ii) a CD prevalence study, by random clustered sampling and proportionate stratification of various geographical areas in Greece. Trained nonmedical staff performed a rapid immunochromatographic test to detect IgA antibodies to tTG-IgA and IgA deficiency. Toddlers with positive results were referred to a paediatric gastroenterologist for further assessment with serum anti-tTG IgA and EMA-IgA. Children with positive serum anti-tTG and anti-EMA underwent upper gastrointestinal tract endoscopy and small bowel biopsy and were subsequently in gluten-free diet. Results In this project participated 1136 toddlers, who were tested at school. The prevalence of positive rapid anti-tTG screening was 1:154, of IgA deficiency 1:120 and of biopsy-proven CD 1:154. The prevalence of CD from this pilot study served as expected prevalence value for sample size calculation for the main prevalence study. Conclusion This protocol using rapid immunochromatographic test for the detection of both IgA deficiency and CD is easy to be performed by nonmedical staff in a community setting, enabling the accurate identification of new CD cases among asymptomatic population.
AB - Aim Evaluation of the prevalence of coeliac disease (CD) in Greek paediatric population. Methods The project consists of two parts: (i) a pilot study of preschool children aged 2-6 years to test the feasibility and diagnostic accuracy of community-based screening and (ii) a CD prevalence study, by random clustered sampling and proportionate stratification of various geographical areas in Greece. Trained nonmedical staff performed a rapid immunochromatographic test to detect IgA antibodies to tTG-IgA and IgA deficiency. Toddlers with positive results were referred to a paediatric gastroenterologist for further assessment with serum anti-tTG IgA and EMA-IgA. Children with positive serum anti-tTG and anti-EMA underwent upper gastrointestinal tract endoscopy and small bowel biopsy and were subsequently in gluten-free diet. Results In this project participated 1136 toddlers, who were tested at school. The prevalence of positive rapid anti-tTG screening was 1:154, of IgA deficiency 1:120 and of biopsy-proven CD 1:154. The prevalence of CD from this pilot study served as expected prevalence value for sample size calculation for the main prevalence study. Conclusion This protocol using rapid immunochromatographic test for the detection of both IgA deficiency and CD is easy to be performed by nonmedical staff in a community setting, enabling the accurate identification of new CD cases among asymptomatic population.
KW - Children
KW - Coeliac disease
KW - Screening
KW - Tissue transglutaminase IgA
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U2 - 10.1111/apa.12241
DO - 10.1111/apa.12241
M3 - Article
C2 - 23600795
AN - SCOPUS:84878545760
SN - 0803-5253
VL - 102
SP - 749
EP - 754
JO - Acta Paediatrica, International Journal of Paediatrics
JF - Acta Paediatrica, International Journal of Paediatrics
IS - 7
ER -