Scanning all-fiber-optic endomicroscopy system for 3D nonlinear optical imaging of biological tissues

Yicong Wu, Yuxin Leng, Jiefeng Xi, Xingde Li

Research output: Contribution to journalArticlepeer-review

131 Scopus citations


An extremely compact all-fiber-optic scanning endomicroscopy system was developed for two-photon fluorescence (TPF) and second harmonic generation (SHG) imaging of biological samples. A conventional double-clad fiber (DCF) was employed in the endomicroscope for single-mode femtosecond pulse delivery, multimode nonlinear optical signals collection and fast two-dimensional scanning. A single photonic bandgap fiber (PBF) with negative group velocity dispersion at two-photon excitation wavelength (i.e. ∼810 nm) was used for pulse prechirping in replacement of a bulky grating/lens-based pulse stretcher. The combined use of DCF and PBF in the endomicroscopy system made the endomicroscope basically a plug-and-play unit. The excellent imaging ability of the extremely compact all-fiber-optic nonlinear optical endomicroscopy system was demonstrated by SHG imaging of rat tail tendon and depth-resolved TPF imaging of epithelial tissues stained with acridine orange. The preliminary results suggested the promising potential of this extremely compact all-fiber-optic endomicroscopy system for realtime assessment of both epithelial and stromal structures in luminal organs.

Original languageEnglish (US)
Pages (from-to)7907-7915
Number of pages9
JournalOptics Express
Issue number10
StatePublished - May 11 2009

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics


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