Saturating the region of the polycystic kidney disease gene with NotI linking clones

Heinz Himmelbauer, Greg G. Germino, Isabella Ceccherini, Giovanni Romeo, Stephen T. Reeders, Anna Maria Frischauf

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

A NotI-linking library was constructed from a radiation hybrid containing fragments of human chromosome 16. The clones were mapped on a panel of somatic cell hybrids, and 10 different NotI site-containing clones were localized close to and between genetic markers flanking the PKD1 locus. With pulsed-field gel analysis the clones were shown to be distributed over four adjacent ClaI fragments covering 1,200 kb.

Original languageEnglish (US)
Pages (from-to)325-334
Number of pages10
JournalAmerican Journal of Human Genetics
Volume48
Issue number2
StatePublished - Feb 1991
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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