Sanger dideoxy sequencing of DNA

Sarah E. Walker, Jon Lorsch

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Scopus citations


While the ease and reduced cost of automated DNA sequencing has largely obviated the need for manual dideoxy sequencing for routine purposes, specific applications require manual DNA sequencing. For instance, in studies of enzymes or proteins that bind or modify DNA, a DNA ladder is often used to map the site at which an enzyme is bound or a modification occurs. In these cases, the Sanger method for dideoxy sequencing provides a rapid and facile method for producing a labeled DNA ladder.

Original languageEnglish (US)
Title of host publicationLaboratory Methods in Enzymology
Subtitle of host publicationDNa
PublisherAcademic Press Inc.
Number of pages14
ISBN (Print)9780124186873
StatePublished - 2013

Publication series

NameMethods in Enzymology
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology


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